Research Article

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Isolates of Bipolaris sorokiniana were analyzed by random-amplified polymorphic DNA (RAPD) techniques to determine the amount of intraspecific genetic variability and to study host-pathogen interactions. Ten isolates originated from different regions of Brazil were examined. Plants of the wheat cultivars BR8, BH1146 (original host) and IAC-5 Maringá, classified as resistant ... more

A.T.S. Matsumura; A.M. Prestes; S.T. Van Der Sand; A.M.R. de Oliveira
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Wheat is notorious for callus induction, which is a major hindrance in direct gene transfer and consequently for genetic improvement programs. In order to provide a successful platform for gene transformation, good callus quantity and quality is impor­tant. We investigated the variation in callus induction capabilities of Pakistani wheat cultivars and measured the reducing sugar con­tent ... more

M.M. Shah; Q. Khalid; U.W. Khan; S.A.H. Shah; S.H. Shah; A. Hassan; A. Pervez
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Abiotic stress resistance; Calreticulin; Defense response; Puccinia striiformis; Wheat

Calreticulin proteins play essential roles in regulating various metabolic processes and in molecular signal transduction in animals and plants. Using homologous PCR, we screened a cDNA library of the wheat resistance gene Yr5 from a near-isogenic line in the susceptible common wheat variety Taichung 29, which was inoculated with an incompatible race CYR32 of Puccinia striiformis. We ... more

Y.Q. An; R.M. Lin; F.T. Wang; J. Feng; Y.F. Xu; S.C. Xu
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Conservation; MicroRNAs; Target genes; Wheat

MicroRNAs (miRNAs) are non-coding small RNAs that regulate gene expression by translational repression or transcript degradation. A large number of miRNAs have been identified from model plant species; however, the character of conserved miRNAs is poorly understood. We studied 42 miRNA families that are conserved within the plant kingdom, using the miRBase database. Some conserved miRNA ... more

Z.J. Yin; F.F. Shen
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AFLP; Cytoplasmic male sterility; Molecular marker; mtDNA; Wheat

To produce a good F1 hybrid variety wheat crop, it is necessary to explore novel cytoplasmic male sterility (CMS) lines and their maintainer line. This study aimed to identify cytoplasmic variation in three isonuclear-alloplasmic male sterile lines Aegilops kotschyi (Ae.kots) -90-110, Aegilops ventricosa (Ae.ven) -90-110, and Triticum spelta ( ... more

M. Ejaz; Z. Qidi; Z. Gaisheng; W. Qunzhu; Z. Xinbo
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Pepper seeds (Capsicum annuum L.) var. B12 were mutagenized by four presoaking treatments in ten concentrations of ethyl methane sulfonate (EMS) to determine the sensitivity of the first generation (M1) to mutagens. The spectrum of mutations and induced variability for various quantitative traits, including germination, percent plant height, injury occurrence, survival ratio, ... more

M.H. Arisha; B.K. Liang; S.N.Muhammad Shah; Z.H. Gong; D.W. Li
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Genetic diversity; Landrace; Oryza sativa; Population structure; Simple sequence repeat

This study analyzed 394 Korean rice landrace accessions, including 93 waxy varieties, for polymorphisms using 29 simple sequence repeat (SSR) markers. In total, 381 alleles served as raw data for estimating the genetic diversity (GD) and population structure. The number of alleles per locus ranged from 3 to 44 (average = 13.14). The expected heterozygosity and polymorphism information ... more

F.P. Li; Y.S. Lee; S.W. Kwon; G. Li; Y.J. Park