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2012
D. S. Britto, Pirovani, C. P., Gonzalez, E. R., Silva, J. F., Gesteira, A. S., and Cascardo, J. C. M., Oxidative stress proteins as an indicator of a low quality of eucalyptus clones for the pulp and paper industry, vol. 11, pp. 3798-3813, 2012.
Afzal AJ, Wood AJ and Lightfoot DA (2008). Plant receptor-like serine threonine kinases: roles in signaling and plant defense. Mol. Plant Microbe Interact. 21: 507-517. http://dx.doi.org/10.1094/MPMI-21-5-0507 PMid:18393610   Alscher RG, Erturk N and Heath LS (2002). Role of superoxide dismutases (SODs) in controlling oxidative stress in plants. J. Exp. Bot. 53: 1331-1341. http://dx.doi.org/10.1093/jexbot/53.372.1331 PMid:11997379   Barbosa LCA, Maltha CRA and Cruz MP (2005). Composição química de extrativos lipofílicos e polares de madeira de Eucalyptus grandis. Ciênc. Eng. 15: 13-20.   Blackbourn HD and Battey NH (1993). Annexin-mediated secretory vesicle aggregation in plants. Physiol. Plant 89: 27-32. http://dx.doi.org/10.1111/j.1399-3054.1993.tb01782.x   Brett D, Pospisil H, Valcarcel J, Reich J, et al. (2002). Alternative splicing and genome complexity. Nat. 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O Papel 47: 36-38.   Gonçalves D, Lepikson-Neto J, Salazar M, Nascimento L, et al. (2011). Eucalyptus Transcriptome Analysis Revealed Molecular Chaperones Highly Expressed in Xylem. Proceedings of IUFRO Tree Biotechnology Conference: From Genomes to Integration and Delivery, Arraial D'Ajuda, Julho 26-02. BMC Proc. 5 (Suppl 7): P109.   Grattapaglia D (2007). Marker-Assisted Selection in Eucalyptus. In: Chapter 14, Marker-Assisted Selection: Current Status and Future Perspectives in Crops, Livestock, Forestry and Fish (Guimarães EP, Ruane J, Scherf BD, Sonnino A, et al., eds.). Food and Agriculture Organization of the United Nations, Rome.   Hsu DS, Kim ST, Sun Q and Sancar A (1995). Structure and function of the UvrB protein. J. Biol. Chem. 270: 8319-8327. http://dx.doi.org/10.1074/jbc.270.14.8319 PMid:7713940   Hurkman WJ and Tanaka CK (1986). Solubilization of plant membrane proteins for analysis by two-dimensional gel electrophoresis. Plant Physiol. 81: 802-806. http://dx.doi.org/10.1104/pp.81.3.802 PMid:16664906 PMCid:1075430   Hwang SS, Cheah SC, Kulaveerasingam H and Tan SH (2003). Molecular cloning and characterization of S-adenosylmethionine synthetase isolated from suspension culture cDNA library of oil palm (Elaeis guineensis Jacq.). Pak. J. Biol. Sci. 6: 1468-1475. http://dx.doi.org/10.3923/pjbs.2003.1468.1475   Klemenz R, Frohli E, Steiger RH, Schafer R, et al. (1991). aB-Crystallin is a small heat shock protein. Proc. Natl. Acad. Sci. 88: 3652-3656. http://dx.doi.org/10.1073/pnas.88.9.3652 PMid:2023914 PMCid:51510   Kube PD, Raymond CA and Banham PW (2001). Genetic parameters for diameter, basic density, cellulose content and fibre properties for Eucalyptus nitens. Forest Genet. 8: 285-294.   Laemmli UK (1970). Cleavage of structural proteins during the assembly of the head of bacteriophage T4. Nature 227: 680-685. http://dx.doi.org/10.1038/227680a0 PMid:5432063   Le Provost G, Paiva J, Pot D, Brach J, et al. (2003). Seasonal variation in transcript accumulation in wood-forming tissues of maritime pine (Pinus pinaster Ait.) with emphasis on a cell wall glycine-rich protein. Planta 217: 820-830. http://dx.doi.org/10.1007/s00425-003-1051-2 PMid:12768425   Lonetto M, Gribskov M and Gross CA (1992). The sigma 70 family: sequence conservation and evolutionary relationships. J. Bacteriol. 174: 3843-3849. PMid:1597408 PMCid:206090   Martinoia E, Klein M, Geisler M, Bovet L, et al. (2002). Multifunctionality of plant ABC transporters-more than just detoxifiers. Planta 214: 345-355. http://dx.doi.org/10.1007/s004250100661 PMid:11855639   Morais SAL, Nascimento EA and Melo DC (2005). Análise de madera de Pinus oocarpa parte I: Estudo dos constituintes macromoleculares e extrativos voláteis. Rev. Árvore, Soc. Invest. Florestais 29: 461-470.   Neuhoff V, Arold N, Taube D and Ehrhardt W (1988). Improved staining of proteins in polyacrylamide gels including isoelectric focusing gels with clear background at nanogram sensitivity using Coomassie Brilliant Blue G-250 and R-250. Electrophoresis 9: 255-262. http://dx.doi.org/10.1002/elps.1150090603 PMid:2466658   Pereira H (1988). Variability in the chemical composition of plantation eucalypts (Eucalyptus globulus Labill.). Wood Fiber Sci. 20: 82-90.   Plomion C, Pionneau C, Brach J, Costa P, et al. (2000). Compression wood-responsive proteins in developing xylem of maritime pine (Pinus pinaster ait.). Plant Physiol. 123: 959-969. http://dx.doi.org/10.1104/pp.123.3.959 PMid:10889244 PMCid:59058   Plomion C, Leprovost G and Stokes A (2001). Wood formation in trees. Plant Physiol. 127: 1513-1523. http://dx.doi.org/10.1104/pp.010816 PMid:11743096 PMCid:1540185   Poke FS, Potts BM, Vaillancourt RE and Raymond CA (2006). Genetic parameters for lignin, extractives and decay in Eucalyptus globulus. Ann. Forest Sci. 63: 813-821. http://dx.doi.org/10.1051/forest:2006080   Rengel D, San Clemente H, Servant F, Ladouce N, et al. (2009). A new genomic resource dedicated to wood formation in Eucalyptus. BMC Plant Biol. 9: 36. http://dx.doi.org/10.1186/1471-2229-9-36 PMid:19327132 PMCid:2670833   Roberts MR, Salinas J and Collinge DB (2002). 14-3-3 proteins and the response to abiotic and biotic stress. Plant Mol. Biol. 50: 1031-1039. http://dx.doi.org/10.1023/A:1021261614491 PMid:12516870   Thumma BR, Southerton SG, Bell JC, Owen JV, et al. (2010). Quantitative trait locus (QTL) analysis of wood quality traits in Eucalyptus nitens. Tree Genet. Genomes 6: 305-317. http://dx.doi.org/10.1007/s11295-009-0250-9   Vander Mijnsbrugge K, Meyermans H, Van Montagu M, Bauw G, et al. (2000). Wood formation in poplar: identification, characterization, and seasonal variation of xylem proteins. Planta 210: 589-598. http://dx.doi.org/10.1007/s004250050048 PMid:10787052   Whetten R, Sun Y-H, Zhang Y and Sederoff R (2001). Functional genomics and cell wall biosynthesis in loblolly pine. Plant Mol. Biol. 47: 275-291. http://dx.doi.org/10.1023/A:1010652003395 PMid:11554476   Wright C, Edelmann M, diGleria K, Kollnberger S, et al. (2009). Ankylosing spondylitis monocytes show upregulation of proteins involved in inflammation and the ubiquitin proteasome pathway. Ann. Rheum. Dis. 68: 1626-1632. http://dx.doi.org/10.1136/ard.2008.097204 PMid:18952638   Yang T and Poovaiah BW (2002). Hydrogen peroxide homeostasis: Activation of plant catalase by calcium_calmodulin. PNAS 99: 4097-4102. http://dx.doi.org/10.1073/pnas.052564899 PMid:11891305 PMCid:122654   Ye ZH, Kneusel RE, Matern U and Varner JE (1994). An alternative methylation pathway in lignin biosynthesis in Zinnia. Plant Cell 6: 1427-1439. PMid:7994176 PMCid:160531
J. H. Amorim, Vidal, R. O., Lacerda-Junior, G. V., Dias, J. C. T., Brendel, M., Rezende, R. P., and Cascardo, J. C. M., A simple boiling-based DNA extraction for RAPD profiling of landfarm soil to provide representative metagenomic content, vol. 11, pp. 182-189, 2012.
Amorim JH, Macena TN, Lacerda GV Jr, Rezende RP, et al. (2008). An improved extraction protocol for metagenomic DNA from a soil of the Brazilian Atlantic Rainforest. Genet. Mol. Res. 7: 1226-1232. http://dx.doi.org/10.4238/vol7-4gmr509 PMid:19065757 Ashburner M, Ball CA, Blake JA, Botstein D, et al. (2000). Gene Ontology: tool for the unification of biology. The Gene Ontology Consortium. Nat. Genet. 25: 25-29. http://dx.doi.org/10.1038/75556 PMid:10802651    PMCid:3037419 Daniel R (2004). The soil metagenome - a rich resource for the discovery of novel natural products. Curr. Opin. Biotechnol. 15: 199-204. http://dx.doi.org/10.1016/j.copbio.2004.04.005 PMid:15193327 Ewing B, Hillier L, Wendl MC and Green P (1998). Base-calling of automated sequencer traces using phred. I. Accuracy assessment. Genome Res. 8: 175-185. PMid:9521921 Huson DH, Auch AF, Qi J and Schuster SC (2007). MEGAN analysis of metagenomic data. Genome Res. 17: 377-386. http://dx.doi.org/10.1101/gr.5969107 PMid:17255551    PMCid:1800929 Johnson PL and Slatkin M (2006). Inference of population genetic parameters in metagenomics: a clean look at messy data. Genome Res. 16: 1320-1327. http://dx.doi.org/10.1101/gr.5431206 PMid:16954540    PMCid:1581441 Kanehisa M and Goto S (2000). KEGG: Kyoto encyclopedia of genes and genomes. Nucleic Acids Res. 28: 27-30. http://dx.doi.org/10.1093/nar/28.1.27 PMid:10592173    PMCid:102409 Maciel BM, Dias JC, Dos Santos AC, Argolo Filho RC, et al. (2007). Microbial surfactant activities from a petrochemical landfarm in a humid tropical region of Brazil. Can. J. Microbiol. 53: 937-943. http://dx.doi.org/10.1139/W07-052 PMid:17898850 McHardy AC and Rigoutsos I (2007). What’s in the mix: phylogenetic classification of metagenome sequence samples. Curr. Opin. Microbiol. 10: 499-503. http://dx.doi.org/10.1016/j.mib.2007.08.004 PMid:17933580 Riesenfeld CS, Schloss PD and Handelsman J (2004). Metagenomics: genomic analysis of microbial communities. Annu. Rev. Genet. 38: 525-552. http://dx.doi.org/10.1146/annurev.genet.38.072902.091216 PMid:15568985 Robe P, Nalin R, Capellano C, Vogel TM, et al. (2003). Extraction of DNA from soil. Eur. J. Soil. Biol. 39: 183-190. http://dx.doi.org/10.1016/S1164-5563(03)00033-5 Roh C, Villatte F, Kim BG and Schmid RD (2006). Comparative study of methods for extraction and purification of enviromental DNA from soil and sludge samples. Appl. Biochem. Biotech. 134: 97-112. http://dx.doi.org/10.1385/ABAB:134:2:97 Rondon MR, August PR, Bettermann AD, Brady SF, et al. (2000). Cloning the soil metagenome: a strategy for accessing the genetic and functional diversity of uncultured microorganisms. Appl. Environ. Microbiol. 66: 2541-2547. http://dx.doi.org/10.1128/AEM.66.6.2541-2547.2000 PMid:10831436    PMCid:110579 Sambrook J, Fritsch EF and Maniatis T (1989). Molecular Cloning: A Laboratory Manual. 3rd edn. Cold Spring Harbor Laboratory Press, New York. Schloss PD and Handelsman J (2003). Biotechnological prospects from metagenomics. Curr. Opin. Biotechnol. 14: 303-310. http://dx.doi.org/10.1016/S0958-1669(03)00067-3 Smit AFA, Hubley R and Green P (2004). RepeatMasker Open-3.0. 1996-2007. Streit WR and Schmitz RA (2004). Metagenomics - the key to the uncultured microbes. Curr. Opin. Microbiol. 7: 492-498. http://dx.doi.org/10.1016/j.mib.2004.08.002 PMid:15451504 Yun J, Kang S, Park S, Yoon H, et al. (2004). Characterization of a novel amylolytic enzyme encoded by a gene from a soil-derived metagenomic library. Appl. Environ. Microbiol. 70: 7229-7235. http://dx.doi.org/10.1128/AEM.70.12.7229-7235.2004 PMid:15574921    PMCid:535135
2010
M. A. Lopes, Júnior, B. T. Hora, Júnior, B. T. Hora, Dias, C. V., Santos, G. C., Gramacho, K. P., Cascardo, J. C. M., Gesteira, A. S., and Micheli, F., Expression analysis of transcription factors from the interaction between cacao and Moniliophthora perniciosa (Tricholomataceae), vol. 9, pp. 1279-1297, 2010.
Andebrhan T, Figueira A, Yamada MM, Cascardo J, et al. (1999). Molecular fingerprinting suggests two primary outbreaks of witches' broom disease (Crinipellis perniciosa) of Theobroma cacao in Bahia, Brazil. Eur. J. Plant Pathol. 105: 167-175. http://dx.doi.org/10.1023/A:1008716000479   Bailey BA, Bae H, Strem MD, Antunez de MG, et al. (2005a). Developmental expression of stress response genes in Theobroma cacao leaves and their response to Nep1 treatment and a compatible infection by Phytophthora megakarya. Plant Physiol. Biochem. 43: 611-622. http://dx.doi.org/10.1016/j.plaphy.2005.04.006 PMid:15979314   Bailey BA, Strema MD, Bae H, Mayolo GA, et al. (2005b). Gene expression in leaves of Theobroma cacao in response to mechanical wounding, ethylene, and/or methyl jasmonate. Plant Sci. 168: 1247-1258. http://dx.doi.org/10.1016/j.plantsci.2005.01.002   Borrone JW, Kuhn DN and Schnell RJ (2004). Isolation, characterization, and development of WRKY genes as useful genetic markers in Theobroma cacao. Theor. Appl. Genet. 109: 495-507. http://dx.doi.org/10.1007/s00122-004-1662-4 PMid:15148571   Brown JS, Phillips-Mora W, Power EJ, Krol C, et al. (2007). Mapping QTLs for resistance to frosty pod and black pod diseases and horticultural traits in Theobroma cacao L. Crop Sci. 47: 1851-1858. http://dx.doi.org/10.2135/cropsci2006.11.0753   Ceita GO, Macêdo JNA, Santos TB, Alemanno L, et al. (2007). Involvement of calcium oxalate degradation during programmed cell death in Theobroma cacao tissues triggered by the hemibiotrophic fungus Moniliophthora perniciosa. Plant Sci. 173: 106-117. http://dx.doi.org/10.1016/j.plantsci.2007.04.006   Century K, Reuber TL and Ratcliffe OJ (2008). Regulating the regulators: the future prospects for transcription-factor-based agricultural biotechnology products. Plant Physiol. 147: 20-29. http://dx.doi.org/10.1104/pp.108.117887 PMid:18443103 PMCid:2330319   Dong J, Chen C and Chen Z (2003). Expression profiles of the Arabidopsis WRKY gene superfamily during plant defense response. Plant Mol. Biol. 51: 21-37. http://dx.doi.org/10.1023/A:1020780022549 PMid:12602888   Eisen MB, Spellman PT, Brown PO and Botstein D (1998). Cluster analysis and display of genome-wide expression patterns. Proc. Natl. Acad. Sci U. S. A. 95: 14863-14868. http://dx.doi.org/10.1073/pnas.95.25.14863 PMid:9843981 PMCid:24541   Eulgem T (2005). Regulation of the Arabidopsis defense transcriptome. Trends Plant Sci. 10: 71-78. http://dx.doi.org/10.1016/j.tplants.2004.12.006 PMid:15708344   Eulgem T and Somssich IE (2007). Networks of WRKY transcription factors in defense signaling. Curr. Opin. Plant Biol. 10: 366-371. http://dx.doi.org/10.1016/j.pbi.2007.04.020 PMid:17644023   Frias GA, Purdy LH and Schmidt RA (1995). An inoculation method for evaluating resistance of cacao to Crinipellis perniciosa. Plant Dis. 79: 787-791. http://dx.doi.org/10.1094/PD-79-0787   Ganesh D, Petitot A-S, Silva MC, Alary R, et al. (2006). Monitoring of the early molecular resistance responses of coffee (Coffea arabica L.) to the rust fungus (Hemileia vastatrix) using real-time quantitative RT-PCR. Plant Sci. 170: 1045-1051. http://dx.doi.org/10.1016/j.plantsci.2005.12.009   Gesteira AS, Micheli F, Ferreira CF and Cascardo JC (2003). Isolation and purification of functional total RNA from different organs of cacao tree during its interaction with the pathogen Crinipellis perniciosa. Biotechniques 35: 494-500.   Gesteira AS, Micheli F, Carels N, Da Silva AC, et al. (2007). Comparative analysis of expressed genes from cacao meristems infected by Moniliophthora perniciosa. Ann. Bot. 100: 129-140. http://dx.doi.org/10.1093/aob/mcm092 PMid:17557832 PMCid:2735303   Gurr SJ and Rushton PJ (2005a). Engineering plants with increased disease resistance: what are we going to express? Trends Biotechnol. 23: 275-282. http://dx.doi.org/10.1016/j.tibtech.2005.04.007 PMid:15922079   Gurr SJ and Rushton PJ (2005b). Engineering plants with increased disease resistance: how are we going to express it? Trends Biotechnol. 23: 283-290. http://dx.doi.org/10.1016/j.tibtech.2005.04.009 PMid:15922080   Jalali BL, Bhargava S and Kamble A (2006). Signal transduction and transcriptional regulation of plant defense responses. J. Phytopathol. 154: 65-74. http://dx.doi.org/10.1111/j.1439-0434.2006.01073.x   Journot-Catalino N, Somssich IE, Roby D and Kroj T (2006). The transcription factors WRKY11 and WRKY17 act as negative regulators of basal resistance in Arabidopsis thaliana. Plant Cell 18: 3289-3302. http://dx.doi.org/10.1105/tpc.106.044149 PMid:17114354 PMCid:1693958   Kaminaka H, Nake C, Epple P, Dittgen J, et al. (2006). bZIP10-LSD1 antagonism modulates basal defense and cell death in Arabidopsis following infection. EMBO J. 25: 4400-4411. http://dx.doi.org/10.1038/sj.emboj.7601312 PMid:16957775 PMCid:1570446   Laquitaine L, Gomes E, Francois J, Marchive C, et al. (2006). Molecular basis of ergosterol-induced protection of grape against Botrytis cinerea: induction of type I LTP promoter activity, WRKY, and stilbene synthase gene expression. Mol. Plant Microbe Interact. 19: 1103-1112. http://dx.doi.org/10.1094/MPMI-19-1103 PMid:17022174   Leal GA, Albuquerque PSB and Figueira A (2007). Genes differentially expressed in Theobroma cacao associated with resistance to witches' broom disease caused by Crinipellis perniciosa. Mol. Plant Pathol. 8: 279-292. http://dx.doi.org/10.1111/j.1364-3703.2007.00393.x PMid:20507499   Li G, Osborne J and Asiegbu FO (2006). A macroarray expression analysis of novel cDNAs vital for growth initiation and primary metabolism during development of Heterobasidion parviporum conidiospores. Environ. Microbiol. 8: 1340-1350. http://dx.doi.org/10.1111/j.1462-2920.2006.01027.x PMid:16872398   Liu Y, Schiff M and Dinesh-Kumar SP (2004). Involvement of MEK1 MAPKK, NTF6 MAPK, WRKY/MYB transcription factors, COI1 and CTR1 in N-mediated resistance to tobacco mosaic virus. Plant J. 38: 800-809. http://dx.doi.org/10.1111/j.1365-313X.2004.02085.x PMid:15144381   Lopez F, Rougemont J, Loriod B, Bourgeois A, et al. (2004). Feature extraction and signal processing for nylon DNA microarrays. BMC Genomics 5: 38. http://dx.doi.org/10.1186/1471-2164-5-38 PMid:15222896 PMCid:471548   Marchive C, Mzid R, Deluc L, Barrieu F, et al. (2007). Isolation and characterization of a Vitis vinifera transcription factor, VvWRKY1, and its effect on responses to fungal pathogens in transgenic tobacco plants. J. Exp. Bot. 58: 1999-2010. http://dx.doi.org/10.1093/jxb/erm062 PMid:17456504   Mellway RD, Tran LT, Prouse MB, Campbell MM, et al. (2009). The wound-, pathogen-, and ultraviolet B-responsive MYB134 gene encodes an R2R3 MYB transcription factor that regulates proanthocyanidin synthesis in poplar. Plant Physiol. 150: 924-941. http://dx.doi.org/10.1104/pp.109.139071 PMCid:2689947   Mzid R, Marchive C, Blancard D, Deluc L, et al. (2007). Overexpression of VvWRKY2 in tobacco enhances broad resistance to necrotrophic fungal pathogens. Physiol. Plant 131: 434-447. http://dx.doi.org/10.1111/j.1399-3054.2007.00975.x PMid:18251882   Pandey SP and Somssich IE (2009). The role of WRKY transcription factors in plant immunity. Plant Physiol. 150: 1648- 1655. http://dx.doi.org/10.1104/pp.109.138990 PMid:19420325 PMCid:2719123   Purdy LH and Schmidt RA (1996). Status of cacao witches' broom: biology, epidemiology, and management. Annu. Rev. Phytopathol. 34: 573-594. http://dx.doi.org/10.1146/annurev.phyto.34.1.573 PMid:15012557   Raffaele S, Rivas S and Roby D (2006). An essential role for salicylic acid in AtMYB30-mediated control of the hypersensitive cell death program in Arabidopsis. FEBS Lett. 580: 3498-3504. http://dx.doi.org/10.1016/j.febslet.2006.05.027 PMid:16730712   Rodrigues SM, Soares VL, de Oliveira TM, Gesteira AS, et al. (2007). Isolation and purification of RNA from tissues rich in polyphenols, polysaccharides, and pigments of annatto (Bixa orellana L.). Mol. Biotechnol. 37: 220-224. http://dx.doi.org/10.1007/s12033-007-0070-9 PMid:17952668   Sarowar S, Oh HW, Cho HS, Baek KH, et al. (2007). Capsicum annuum CCR4-associated factor CaCAF1 is necessary for plant development and defence response. Plant J. 51: 792-802. http://dx.doi.org/10.1111/j.1365-313X.2007.03174.x PMid:17587232   Schnell RJ, Kuhn DN, Brown JS, Olano CT, et al. (2007). Development of a marker assisted selection program for cacao. Phytopathology 97: 1664-1669. http://dx.doi.org/10.1094/PHYTO-97-12-1664 PMid:18943731   Silva SDVM, Luz EDMN, Almeida OC, Gramacho K, et al. (2002). Redescrição da sintomatologia causada por Crinipellis perniciosa em cacaueiros. Agrotropica 14: 1-24.   Terrier N, Torregrosa L, Ageorges A, Vialet S, et al. (2009). Ectopic expression of VvMybPA2 promotes proanthocyanidin biosynthesis in grapevine and suggests additional targets in the pathway. Plant Physiol. 149: 1028-1041. http://dx.doi.org/10.1104/pp.108.131862 PMid:19098092 PMCid:2633825   Thurow C, Schiermeyer A, Krawczyk S, Butterbrodt T, et al. (2005). Tobacco bZIP transcription factor TGA2.2 and related factor TGA2.1 have distinct roles in plant defense responses and plant development. Plant J. 44: 100-113. http://dx.doi.org/10.1111/j.1365-313X.2005.02513.x PMid:16167899   Wang E (2005). RNA amplification for successful gene profiling analysis. J. Transl. Med. 3: 28. http://dx.doi.org/10.1186/1479-5876-3-28 PMid:16042807 PMCid:1201175   Yang B, Jiang Y, Rahman MH, Deyholos MK, et al. (2009). Identification and expression analysis of WRKY transcription factor genes in canola (Brassica napus L.) in response to fungal pathogens and hormone treatments. BMC Plant Biol. 9: 68. http://dx.doi.org/10.1186/1471-2229-9-68 PMid:19493335 PMCid:2698848   Yang YH, Dudoit S, Luu P, Lin DM, et al. (2002). Normalization for cDNA microarray data: a robust composite method addressing single and multiple slide systematic variation. Nucleic Acids Res. 30: e15. http://dx.doi.org/10.1093/nar/30.4.e15 PMid:11842121 PMCid:100354