Wistar-Imamichi rat

Cloning, sequencing, and polymorphisms of the Wistar-Imamichi rat growth hormone gene using PCR-SSCP

M. Q. Liu, Wang, J. G., and Li, X. L., Cloning, sequencing, and polymorphisms of the Wistar-Imamichi rat growth hormone gene using PCR-SSCP, vol. 12, pp. 6203-6211, 2013.

We successfully cloned the Wistar-Imamichi (WI) rat growth hormone gene (GenBank accession: GQ890681), which contained 5 exons and 4 introns. Using the polymerase chain reaction-single-strand conformation polymorphism (PCR-SSCP) technique, a novel missense substitution single nucleotide polymorphism was identified and tested for Hardy-Weinberg equilibrium with the χ2 test. This gene fragment was investigated in 50 adult rats and 50 neonatal rats by PCR-SSCP.

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