Wild olives

RAPD assay of wild-type olives in Turkey

M. Sesli and Yegenoglu, E. D., RAPD assay of wild-type olives in Turkey, vol. 9, pp. 966-972, 2010.

Genetic similarities and distances between wild-type olives in Turkey were studied using an RAPD-PCR assay. Seven wild olive tree samples were collected from villages in Manisa and Izmir provinces. Genomic DNA was extracted from young leaves and the RAPD-PCR assay was used to generate RAPD markers. Sixty-five random primers obtained from Operon Technologies were tested for the assay (OP-A 1-20, OP-I 1-20, OP-Q 1-20, and OP-J 1-5). Thirty-two of these primers yielded 115 highly polymorphic bands. The mean number of usable bands per primer for all the samples was 3.59.

Comparison of similarity coefficients used for cluster analysis based on RAPD markers in wild olives

M. Sesli and Yegenoglu, E. D., Comparison of similarity coefficients used for cluster analysis based on RAPD markers in wild olives, vol. 9, pp. 2248-2253, 2010.

Five different similarity coefficients (Jaccard, Sorensen-Dice, simple matching, Rogers and Tanimoto, and Russel and Rao) were evaluated and 10 wild olives analyzed with RAPD markers. The influence of the similarity coefficients on wild olives clustering was investigated. Forty-five primers were used on samples from 10 wild olives (Wild 1 and 2 obtained from Mugla province; Wild 3, 4, 5, 6, 7, and 8 from Manisa province and Wild 9 and 10 from Izmir province of Turkey). The similarity matrices obtained from RAPD markers were compared by the Mantel test.

Subscribe to Wild olives