Preparation of polyclonal antibody against porcine beta defensin 2 and identification of its distribution in tissues of pig
“Preparation of polyclonal antibody against porcine beta defensin 2 and identification of its distribution in tissues of pig”, vol. 14, pp. 18863-18871, 2015.
, Porcine β-defensin 2 (pBD2) is an antimicrobial peptide in pigs that plays an important role in the immune system by preventing bacterial invasion. To produce an anti-pBD2 antibody, which is not commercially available, we expressed and purified a soluble, his-tagged version of pBD2 (his-pBD2). Purified pBD2 was injected into New Zealand white rabbits to generate polyclonal antiserum. Anti-pBD2 antibodies were purified by ammonium sulfate precipitation, followed by diethylaminoethyl cellulose ion-exchange chromatography.