Western blot

Molecular characterization and expression analysis of the Lrh-1 gene in Chinese Hu sheep

L. H. Wang, Zhang, W., Ji, J. L., Gao, Q. X., Xiao, S. H., and Wang, F., Molecular characterization and expression analysis of the Lrh-1 gene in Chinese Hu sheep, vol. 12, pp. 1490-1500, 2013.

Liver receptor homologue 1 (Lrh-1) is a member of the nuclear receptor belonging to the second subfamily of the nuclear receptor family 5A (NR5A), also named NR5A2, which is important for lipid homeostasis, embryogenesis, and regulation of aromatics. The present study aimed to understand the sequence of ovine Lrh-1 and the expression traits in reproductive organ tissues. Initially, we cloned Lrh-1 from the liver of Hu sheep through degenerate primer of reverse transcription-polymerase chain reaction and rapid amplification of cDNA ends.

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