In vitro culture

Characteristics of mature wheat embryos with different resistance to scab cultured in vitro with Fusarium graminearum crude toxin

S. Wang, Wang, M., and Zhang, C. Y., Characteristics of mature wheat embryos with different resistance to scab cultured in vitro with Fusarium graminearum crude toxin, vol. 14, pp. 17348-17357, 2015.

Five wheat varieties with different resistance to scab were used to study the effect of Fusarium graminearum crude toxin on culture characteristics of mature wheat embryos in vitro. The results showed that the rate of induction and differentiation of mature embryo callus differed significantly between varieties, toxin concentration, and concentration x varieties. A low concentration (5 g/L) of F.

Performance of peanut mutants and their offspring generated from mixed high-energy particle field radiation and tissue culture

J. S. Wang, Qiao, L. X., Zhao, L. S., Wang, P., Guo, B. T., Liu, L. X., and Sui, J. M., Performance of peanut mutants and their offspring generated from mixed high-energy particle field radiation and tissue culture, vol. 14, pp. 10837-10848, 2015.

To develop new ways to breed peanut, we irradiated seeds of the Luhua 11 cultivar with a mixed high-energy particle field at different doses. The embryonic leaflets were extracted as explants and incubated on somatic embryo induction medium and then on somatic embryo germination and regeneration medium. After being grafted, the M1-generation plants were transplanted, and seeds from each M1-generation plant were harvested. In the following year, the M2-generation seeds were planted separately.

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