In vitro

Genetic stability of micropropagated plants of Crambe abyssinica Hochst using ISSR markers

E. T. Werner, Soares, T. C. B., Gontijo, A. B. P. L., Neto, J. D. Souza, and Amaral, J. A. Tdo, Genetic stability of micropropagated plants of Crambe abyssinica Hochst using ISSR markers, vol. 14, pp. 16450-16460, 2015.

Crambe (Crambe abyssinica) is a non-edible annual herb, which was first cultivated to extract oil for industry, and now has great potential for biodiesel production. The objective of this investigation was to evaluate the genetic stability of micropropagated plants of the C. abyssinica Hochst cultivar ‘FMS brilhante’ using polymerase chain reaction techniques based on inter-simple sequence repeat (ISSR) molecular markers.

Evaluation of Hsp47 expression in heat-stressed rat myocardial cells in vitro and in vivo

A. Abdelnasir, Sun, J. R., Cheng, Y. F., Chen, H. B., Tang, S., Kemper, N., Hartung, J., and Bao, E. D., Evaluation of Hsp47 expression in heat-stressed rat myocardial cells in vitro and in vivo, vol. 13, pp. 10787-10802, 2014.

The aim of the present study was to identify the correlation between expression of heat shock protein 47 (Hsp47) and stress injury in heat-stressed myocardial cells and to compare variations in Hsp47 expression in rat myocardial cells exposed to different heat stress for varying periods in vitro and in vivo. Exposure to heat stress at 42°C resulted in similar induction patterns of the heart damage-related enzyme aspartate aminotransferase in the supernatants of H9c2 cells and in the serum of rats.

Hsp110 expression changes in rat primary myocardial cells exposed to heat stress in vitro

Z. J. Liu, Lv, Y. J., Zhang, M., Yue, Z. H., Tang, S., Islam, A., Rehana, B., Bao, E. D., and Hartung, J., Hsp110 expression changes in rat primary myocardial cells exposed to heat stress in vitro, vol. 11, pp. 4728-4738, 2012.

We investigated and described the kinetics of heat shock protein (Hsp) 110 expression and distribution in rat primary myocardial cells exposed to heat stress in vitro. After incubation at 37°C for 72 h, myocardial cells were heat stressed at 42°C for 0, 10, 20, 40, 60, 120, 240, 360, and 480 min. Significant increases in aspartate transaminase, lactate dehydrogenase, and creatine kinase enzymatic activities in the myocardial cell culture media were observed during heat stress, suggesting that the integrity of the myocardial cells was altered.

Subscribe to In vitro