Characterization of a novel Cry8Ea3-binding V-ATPase Subunit A in Holotrichia parallela
“Characterization of a novel Cry8Ea3-binding V-ATPase Subunit A in Holotrichia parallela”, vol. 15, p. -, 2016.
, Several receptor proteins of Cry toxin have been previously identified, including cadherin-like, aminopeptidase N, and alkaline phosphatase. In the present work, a novel binding protein, V-ATPase subunit A (HpVAA), was identified in Holotricia parallela larvae and characterized. We performed reverse transcription-polymerase chain reaction and rapid amplification of cDNA ends technology to obtain the cDNA of the full-length hpvaa. Sequencing analysis showed that the open reading frame of hpvaa (GenBank accession No.