Nanofibers promote HepG2 aggregate formation and cellular function
“Nanofibers promote HepG2 aggregate formation and cellular function”, vol. 15, p. -, 2016.
, Formation of hepatocyte spheroids is a necessary strategy for increasing liver-specific function in vitro. In this study, HepG2 cells showed good viability when grown on a polylactic acid-chitosan (PLA-CS) nanofiber and aggregated to form multicellular spheroids on the PLA-CS nanofibers with a diameter of approximately 100-200 mm in 5 days of culture, whereas no such aggregation was observed in cells cultured on 24-well plates.