Ultrasound

Enhancement of recombinant adeno-associated virus mediated transgene expression by targeted echo-contrast agent

S. L. Yang, Mu, Y. M., Tang, K. Q., Jiang, X. K., Bai, W. K., Shen, E., and Hu, B., Enhancement of recombinant adeno-associated virus mediated transgene expression by targeted echo-contrast agent, vol. 12, pp. 1318-1326, 2013.

Ultrasound-targeted microbubble destruction (UTMD) has been recently developed for destroying bubbles carrying drugs or genes, thereby permitting local release of these target molecules. We in­vestigated whether SonoVue®, a new contrast agent that contains phos­pholipid-stabilized microbubbles filled with sulfur hexafluoride vapor, is effective at delivering a recombinant adeno-associated viral (rAAV) vector to the rat heart by UTMD.

Overexpression of HSP27 in cultured human aortic smooth muscular cells reduces apoptosis induced by low-frequency and low-energy ultrasound by inhibition of an intrinsic pathway

B. Zhang, Zhou, H. S., Cheng, Q., Lei, L., and Hu, B., Overexpression of HSP27 in cultured human aortic smooth muscular cells reduces apoptosis induced by low-frequency and low-energy ultrasound by inhibition of an intrinsic pathway, vol. 12, pp. 6588-6601, 2013.

We investigated in vitro the effect of low-frequency and low-energy ultrasound (LFLEU) on apoptosis of an overexpressed HSP27 human aortic smooth muscle cell (HASMC) line. A frequency of 42.6 kHz was used in all experiments. HASMC were exposed to ultrasound and cell viability was evaluated by MTT reduction. Overexpressed HSP27-HASMC was constructed on a pcDNA3.1 vector. Apoptosis was determined 24 h after treatment by flow cytometry; gene display was evaluated with Affimax chips, and HSP27 mRNA and protein expression levels were measured by RT-PCR and Western blotting.

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