Two-dimensional gel electrophoresis

Comparative proteomic changes of differentially expressed whey proteins in clinical mastitis and healthy yak cows

X. Li, Ding, X. Z., Wan, Y. L., Liu, Y. M., and Du, G. Z., Comparative proteomic changes of differentially expressed whey proteins in clinical mastitis and healthy yak cows, vol. 13, pp. 6593-6601, 2014.

Under the traditional grazing system on the Qinghai-Tibetan Plateau, the amount of milk in domesticated yak (Bos grunniens) with clinical mastitis decreases and the milk composition is altered. To understand the mechanisms of mammary gland secreted milk and disease infection, changes in the protein composition of milk during clinical mastitis were investigated using a proteomic approach. Milk whey from yak with clinical mastitis was compared to whey from healthy animals with two-dimensional gel electrophoresis using a mass spectrometer.

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