Two-dimensional electrophoresis

Selecting the optimum conditions for two-dimensional difference gel electrophoresis of proteins expressed in Populus euphratica

J. Q. Hao, Yue, N., Zheng, C. X., Hao, J. Q., Yue, N., and Zheng, C. X., Selecting the optimum conditions for two-dimensional difference gel electrophoresis of proteins expressed in Populus euphratica, vol. 15, p. -, 2016.

We selected the optimum conditions for two-dimensional difference gel electrophoresis (2D-DIGE) of proteins expressed in the heteromorphic leaves of Populus euphratica Oliv. by adjusting the isoelectric focusing, the loading quantity, the concentration of the electrophoretic gel, and other parameters. The results of our study showed that protein separation was improved with many clear protein spots observed, and the differentiations were obvious. The findings of this study will be useful for future studies of protein expression in the heteromorphic leaves of P.

Proteome analysis of tobacco leaves reveals dynamic changes in protein expression among different cultivation areas

L. M. Lu, Ye, K. Y., Tang, Z. X., Liu, L., Chen, Y., Luo, J., and Huang, Y. B., Proteome analysis of tobacco leaves reveals dynamic changes in protein expression among different cultivation areas, vol. 14, pp. 15276-15284, 2015.

The leaves of tobacco plants were used to analyze differences in protein content of tobacco grown in the four main flue-cured tobacco-producing areas of Sichuan Province, China. An improved protein extraction method, isoelectric focusing/sodium dodecyl sulfate-polyacrylamide gel electrophoresis two-dimensional gel electrophoretic separation, was used to extract and separate total protein from tobacco leaves. Proteomic maps with relatively high resolution and repeatability were produced.

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