TSA

Promoter methylation and histone modifications affect the expression of the exogenous DsRed gene in transgenic goats

M. T. Nuo, Yuan, J. L., Yang, W. L., Gao, X. Y., He, N., Liang, H., Cang, M., Liu, D. J., Nuo, M. T., Yuan, J. L., Yang, W. L., Gao, X. Y., He, N., Liang, H., Cang, M., and Liu, D. J., Promoter methylation and histone modifications affect the expression of the exogenous DsRed gene in transgenic goats, vol. 15, p. -, 2016.

Transgene silencing, which is common in transgenic plants and animals, limits the generation and application of genetically modified organisms, and is associated with the exogenous gene copy number, the methylation status of its promoters, and histone modification abnormalities. Here, we analyzed the expression of the exogenous gene DsRed and the methylation status of its cytomegalovirus (CMV) promoter in six healthy transgenic cashmere goats and transgenic nuclear donor cells.

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