Triticum aestivum

Genetic divergence for high-molecular weight glutenin subunits (HMW-GS) in indigenous landraces and commercial cultivars of bread wheat of Pakistan

F. Yasmeen, Khurshid, H., and Ghafoor, A., “Genetic divergence for high-molecular weight glutenin subunits (HMW-GS) in indigenous landraces and commercial cultivars of bread wheat of Pakistan”, vol. 14, pp. 4829-4839, 2015.

Wheat flour quality is an important consideration in the breeding and development of new cultivars. A strong association between high-molecular weight glutenin subunits (HMW-GS) and bread making quality has resulted in the widespread utilization of HMW-GS in wheat breeding. In this study, we analyzed 242 lines of wheat, including landraces from the provinces of Punjab and Baluchistan, as well as the commercial varieties of Pakistan, to determine allelic variation in the Glu-A1, Glu-B1, and Glu-D1 loci encoding HMW-GS.

Cytogenetic and molecular identification of a wheat-Leymus mollis alien multiple substitution line from octoploid Tritileymus x Triticum durum

Y. H. Pang, Zhao, J. X., Du, W. L., Li, Y. L., Wang, J., Wang, L. M., Wu, J., Cheng, X. N., Yang, Q. H., and Chen, X. H., “Cytogenetic and molecular identification of a wheat-Leymus mollis alien multiple substitution line from octoploid Tritileymus x Triticum durum”, vol. 13, pp. 3903-3913, 2014.

Leymus mollis (Trin.) Pilger (NsNsXmXm, 2n = 28), a wild relative of common wheat, possesses many traits that are potentially valuable for wheat improvement. In order to exploit and utilize the useful genes of L. mollis, we developed a multiple alien substitution line, 10DM50, from the progenies of octoploid Tritileymus M842-16 x Triticum durum cv. D4286.

Comparison of small scale methods for the rapid and efficient extraction of mitochondrial DNA from wheat crop suitable for down-stream processes

M. Ejaz, Qidi, Z., Gaisheng, Z., Qunzhu, W., Na, N., and Huiyan, Z., “Comparison of small scale methods for the rapid and efficient extraction of mitochondrial DNA from wheat crop suitable for down-stream processes”, vol. 13, pp. 10320-10331, 2014.

We evaluated and compared 2 mitochondrial DNA (mtDNA) extraction methods in terms of DNA quality and success of subsequent polymerase chain reaction (PCR) amplifications from yel­low etiolated shoots of wheat crop (Triticum aestivum). mtDNA ex­traction is difficult because the presence of metabolites interfere with DNA isolation procedures and downstream applications such as DNA restriction, amplification, and cloning.

Examination of polymorphism in hybrid wheat by using amplified fragment length polymorphism marker

M. Ejaz, Gaisheng, Z., Quidi, Z., Qunzhu, W., and Huiyan, Z., “Examination of polymorphism in hybrid wheat by using amplified fragment length polymorphism marker”, vol. 13, pp. 10076-10085, 2014.

To investigate the polymorphism of two groups of male sterile lines and their maintainer lines, we used amplified fragment length polymorphism markers. Of the 64 primer pairs, five reproducibly demonstrated polymorphism. Group I had 27 polymorphic bands, and Group II had 24 polymorphic bands. The maximum number of polymorphic bands was produced by line 2 (18 polymorphic bands) followed by line 7 (15 polymorphic bands).

Impact of forage clipping treatments on performance of winter wheat

S. A. Jadoon, Ullah, H., Mohammad, F., Khalil, I. H., Alam, M., Shahwar, D., Malik, M. F. A., and Jamal, Y., “Impact of forage clipping treatments on performance of winter wheat”, vol. 12, pp. 5283-5288, 2013.

Farmers in northern parts of Pakistan face severe shortage of green forage for their livestock during the harsh winter season. Winter wheat has the potential to be used as a dual-purpose crop for forage plus grain production in these areas. Ten elite winter wheat lines from Oklahoma State University were evaluated at Hazara Research Station Abbottabad under unclipped and clipped treatment level during 2005-06. The material was planted in a randomized complete block design with three replications, with a row length of four meters and a row to row space of 25 cm.

Molecular characterization of wheat germplasm using microsatellite markers

S. Ijaz and Khan, I. A., “Molecular characterization of wheat germplasm using microsatellite markers”, vol. 8, pp. 809-815, 2009.

We investigated the genetic diversity of 63 wheat genotypes, composed of 48 accessions and 15 varieties, using 56 polymorphic simple sequence repeat primers. One hundred and eighty-six loci were found, with a mean of 131.26 alleles per locus. Cluster analysis based on microsatellite allelic diversity discrimi­nated the accessions and varieties into different clusters; genetic di­versity was the highest between variety Kohistan-97 and accession number 011512, giving a genetic similarity value of 0.4198.

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