Triticum aestivum

Comparison of small scale methods for the rapid and efficient extraction of mitochondrial DNA from wheat crop suitable for down-stream processes

M. Ejaz, Qidi, Z., Gaisheng, Z., Qunzhu, W., Na, N., and Huiyan, Z., Comparison of small scale methods for the rapid and efficient extraction of mitochondrial DNA from wheat crop suitable for down-stream processes, vol. 13, pp. 10320-10331, 2014.

We evaluated and compared 2 mitochondrial DNA (mtDNA) extraction methods in terms of DNA quality and success of subsequent polymerase chain reaction (PCR) amplifications from yel­low etiolated shoots of wheat crop (Triticum aestivum). mtDNA ex­traction is difficult because the presence of metabolites interfere with DNA isolation procedures and downstream applications such as DNA restriction, amplification, and cloning.

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