Trichoderma harzianum

Recombinant expression and characterization of an endoglucanase III (cel12a) from Trichoderma harzianum (Hypocreaceae) in the yeast Pichia pastoris

W. C. Generoso, Malagó-Jr., W., Pereira, Jr., N., and Henrique-Silva, F., Recombinant expression and characterization of an endoglucanase III (cel12a) from Trichoderma harzianum (Hypocreaceae) in the yeast Pichia pastoris, vol. 11, pp. 1544-1557, 2012.

Filamentous fungi from the genus Trichoderma have been widely investigated due to their considerable production of important biotechnological enzymes. Previous studies have demonstrated that the T. harzianum strain IOC-3844 has a high degree of cellulolytic activity. After excluding the native signal peptide, the open reading frame of the T. harzianum endoglucanase III enzyme was cloned in the expression vector pPICZαA, enabling protein secretion to the culture medium. The recombinant plasmid was used to transform Pichia pastoris.

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