Trees

A set of novel microsatellite markers developed for an economically important tree, Dracontomelon duperreanum, in China.

Z. F. Wang, Cao, H. L., Wu, L. F., Guo, Y., Mei, Q. M., Li, M., Wang, Y., and Wang, Z. M., “A set of novel microsatellite markers developed for an economically important tree, Dracontomelon duperreanum, in China.”, Genet Mol Res, vol. 16, no. 2, 2017.

Myrciaria dubia, an Amazonian fruit: population structure and its implications for germplasm conservation and genetic improvement.

C. F. Nunes, Setotaw, T. A., Pasqual, M., Chagas, E. A., Santos, E. G., Santos, D. N., Lima, C. G. B., and Cançado, G. M. A., “Myrciaria dubia, an Amazonian fruit: population structure and its implications for germplasm conservation and genetic improvement.”, Genet Mol Res, vol. 16, no. 1, 2017.

An efficient protocol for tissue sampling and DNA isolation from the stem bark of Leguminosae trees

R. M. L. Novaes, Rodrigues, J. G., and Lovato, M. B., “An efficient protocol for tissue sampling and DNA isolation from the stem bark of Leguminosae trees”, vol. 8, pp. 86-96, 2009.

Traditionally, molecular studies of plant species have used leaves as the source of DNA. However, sampling leaves from tall tree species can be quite difficult and expensive. We developed a sequence of procedures for using stem bark as a source of DNA from Leguminosae trees of the Atlantic Forest and the Cerrado. Leguminosae is an important species-rich family in these two highly diverse and endangered biomes. A modified CTAB protocol for DNA isolation is described, and details of the procedures for sampling and storage of the bark are given.

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