rbcS SRS4 promoter from Glycine max and its expression activity in transgenic tobacco
“rbcS SRS4 promoter from Glycine max and its expression activity in transgenic tobacco”, vol. 14, pp. 7395-7405, 2015.
, The regulatory region of the ribulose-1,5-bisphosphate carboxylase small subunit gene SRS4 from soybean (Glycine max) was cloned using TAIL-PCR and general PCR, and named the rbcS promoter. The promoter was fused with the GUS gene and introduced into Nicotiana tabacum via Agrobacterium-mediated leaf disk transformation. In 4-week-old transgenic tobacco plants, the highest GUS expression levels were observed in the leaves, GUS activity was 7.13- and 7.40-fold higher in leaves than in stems and roots, respectively.