Transgenic tobacco

rbcS SRS4 promoter from Glycine max and its expression activity in transgenic tobacco

X. Y. Cui, Chen, Z. Y., Wu, L., Liu, X. Q., Dong, Y. Y., Wang, F. W., and Li, H. Y., rbcS SRS4 promoter from Glycine max and its expression activity in transgenic tobacco, vol. 14, pp. 7395-7405, 2015.

The regulatory region of the ribulose-1,5-bisphos­phate carboxylase small subunit gene SRS4 from soybean (Glycine max) was cloned using TAIL-PCR and general PCR, and named the rbcS promoter. The promoter was fused with the GUS gene and in­troduced into Nicotiana tabacum via Agrobacterium-mediated leaf disk transformation. In 4-week-old transgenic tobacco plants, the highest GUS expression levels were observed in the leaves, GUS activity was 7.13- and 7.40-fold higher in leaves than in stems and roots, respectively.

Subscribe to Transgenic tobacco