Transformation

Easy detection of green fluorescent protein multicopy transformants in Penicillium griseoroseum

F. J. F. Lopes, de Araújo, E. F., and de Queiroz, M. V., Easy detection of green fluorescent protein multicopy transformants in Penicillium griseoroseum, vol. 3, pp. 449-455, 2004.

Penicillium griseoroseum, a deuteromycete fungus producer of pectinolytic enzymes, was transformed with a gene encoding for green fluorescent protein (GFP). The selection of transformants was based on the homologous nitrate reductase gene (niaD). Protoplasts of a P. griseoroseum Nia mutant (PG63) were co-transformed with the plasmids pNPG1 and pAN52-1-GFP. The plasmid pNPG-1 carries the homologous niaD gene and pAN52-1-GFP carries the SGFP-TYG version of GFP.

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