Effects of BCL2 transfection on the cell cycle and proliferation of human GES-1 cells
“Effects of BCL2 transfection on the cell cycle and proliferation of human GES-1 cells”, vol. 14, pp. 12022-12029, 2015.
, We investigated the effects of BCL2 transfection on the cell cycle and proliferation of GES-1 cells. A pcDNA3-BCL2 plasmid was used to transfect GES-1 cell line human gastric epithelial cells. Clones were obtained by G418 screening. BCL2-positive cells were identified by fluorescence immunohistochemistry. The pcDNA3-BCL2 vectors carrying the NeoR gene were transfected into GES-1 cells, while the empty plasmid was transfected into the same cells as controls. BCL2-positive clones were screened by neomycin 418 (G418).