Transfection

Effect of overexpression of PTEN on apoptosis of liver cancer cells

M. F. Li, Guan, H., Zhang, D. D., Li, M. F., Guan, H., and Zhang, D. D., Effect of overexpression of PTEN on apoptosis of liver cancer cells, vol. 15, p. -, 2016.

Liver cancer is a common malignant tumor associated with a short-survival period and high-mortality rate, and its prevalence in China is particularly high. This study aimed to investigate the effect of overexpressing the phosphatase and tensin homolog deleted on chromosome 10 (PTEN) gene on liver cancer cell apoptosis and provide new insight into the treatment of this disease.

Aldehyde dehydrogenase 2 protects human umbilical vein endothelial cells against oxidative damage and increases endothelial nitric oxide production to reverse nitroglycerin tolerance

X. Y. Hu, Fang, Q., Ma, D., Jiang, L., Yang, Y., Sun, J., Yang, C., Wang, J. S., Hu, X. Y., Fang, Q., Ma, D., Jiang, L., Yang, Y., Sun, J., Yang, C., and Wang, J. S., Aldehyde dehydrogenase 2 protects human umbilical vein endothelial cells against oxidative damage and increases endothelial nitric oxide production to reverse nitroglycerin tolerance, vol. 15, p. -, 2016.

Medical nitroglycerin (glyceryl trinitrate, GTN) use is limited principally by tolerance typified by a decrease in nitric oxide (NO) produced by biotransformation. Such tolerance may lead to endothelial dysfunction by inducing oxidative stress. In vivo studies have demonstrated that aldehyde dehydrogenase 2 (ALDH2) plays important roles in GTN biotransformation and tolerance. Thus, modification of ALDH2 expression represents a potentially effective strategy to prevent and reverse GTN tolerance and endothelial dysfunction.

Molecular cloning and expression vector construction of bovine TRIM28

X. Ma, Zhai, Z. C., Zhang, M. L., Song, B. H., Zhu, Y. R., Yang, S. B., Dong, X. Q., Su, L. Y., Wang, C. F., Ma, H. X., Luan, W. M., Ma, X., Zhai, Z. C., Zhang, M. L., Song, B. H., Zhu, Y. R., Yang, S. B., Dong, X. Q., Su, L. Y., Wang, C. F., Ma, H. X., and Luan, W. M., Molecular cloning and expression vector construction of bovine TRIM28, vol. 15, p. -, 2016.

The bovine TRIM28 gene was amplified from ovary tissue by using RT-PCR. The TRIM28 gene was inserted into the eukaryotic expression vector pIRES2-EGFP and transfected into bovine fetal fibroblasts by using Lipofectamine 3000. TRIM28 mRNA and protein were detected by fluorescence microscope and western blotting. The results showed that the full length of TRIM28 was cloned and pIRES2-EGFP-TRIM28 was constructed successfully. EGFP expression was observed, and the pIRES2-EGFP-TRIM28 transfected group expressed more TRIM28 protein than that by the pIRES2-EGFP group.

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