Transcriptome sequencing

Transcriptome analysis and de novo annotation of the critically endangered Amur sturgeon (Acipenser schrenckii)

X. J. Zhang, Jiang, H. Y., Li, L. M., Yuan, L. H., Chen, J. P., Zhang, X. J., Jiang, H. Y., Li, L. M., Yuan, L. H., and Chen, J. P., Transcriptome analysis and de novo annotation of the critically endangered Amur sturgeon (Acipenser schrenckii), vol. 15, p. -, 2016.

The aim of this study was to provide comprehensive insights into the genetic background of sturgeon by transcriptome study. We performed a de novo assembly of the Amur sturgeon Acipenser schrenckii transcriptome using Illumina Hiseq 2000 sequencing. A total of 148,817 non-redundant unigenes with base length of approximately 121,698,536 bp and ranges from 201 to 26,789 bp were obtained. All the unigenes were classified into 3368 distinct categories and 145,449 singletons by homologous transcript cluster analysis.

Characterization of grass carp spleen transcriptome during GCRV infection

B. H. Xu, Zhong, L., Liu, Q. L., Xiao, T. Y., Su, J. M., Chen, K. J., Wang, H. Q., Dai, Y. J., Chen, J., Xu, B. H., Zhong, L., Liu, Q. L., Xiao, T. Y., Su, J. M., Chen, K. J., Wang, H. Q., Dai, Y. J., Chen, J., Xu, B. H., Zhong, L., Liu, Q. L., Xiao, T. Y., Su, J. M., Chen, K. J., Wang, H. Q., Dai, Y. J., and Chen, J., Characterization of grass carp spleen transcriptome during GCRV infection, vol. 15, p. -, 2016.

The aim of the study was to investigate the grass carp hemorrhagic infection pathway and its key-related genes. Grass carp reovirus (GCRV) might cause hemorrhagic disease in grass carps. Healthy grass carp fingerlings (N = 60) were divided into control and infected groups. Fish in the control group were intraperitoneally (ip) injected with 0.6% fish physiological saline; the infected group received 5,000,000 50% tissue culture infective doses of GCRV 873 standard strain, a double-stranded RNA (dsRNA) virus strain, ip, in 0.5 mL.

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