Transcriptome analysis

Transcriptome analysis of the grass carp (Ctenopharyngodon idella) using 454 pyrosequencing methodology for gene and marker discovery

L. Y. Yu, Bai, J. J., Fan, J. J., Ma, D. M., Quan, Y. C., and Jiang, P., Transcriptome analysis of the grass carp (Ctenopharyngodon idella) using 454 pyrosequencing methodology for gene and marker discovery, vol. 14, pp. 19249-19263, 2015.

Total RNA isolated from the brain, muscle, liver, gonad, and intestinal tissues of grass carp was pooled to construct cDNA libraries. Using 454 pyrosequencing, a total of 738,604 high-quality reads were generated from the normalized cDNAs of the pooled individuals. Clustering and assembly of these reads produced a set of 37,086 all-unigene sequences after BLAST.

Paracoccidioides brasiliensis translation and protein fate machineries revealed by functional genome analysis

D. Paulo de Souza, Silva, S. Souza, Baptista, A. J., Nicola, A. Moraes, Kyaw, C. M., and Silva-Pereira, I., Paracoccidioides brasiliensis translation and protein fate machineries revealed by functional genome analysis, vol. 4, pp. 273-289, 2005.

The translational and post-translational modification machineries of Paracoccidioides brasiliensis were assessed by means of comparative analyses of PbAESTs (P. brasiliensis assembled expressed sequence tags) with sequences deposited on different databases. Of the 79 sequences corresponding to cytosolic ribosomal proteins, we were able to find 78 in the P. brasiliensis transcriptome. Nineteen of the 27 Saccharomyces cerevisiae genes related to translation initiation were also found. All eukaryotic elongation factors were detected in P.

Paracoccidioides brasiliensis RNA biogenesis apparatus revealed by functional genome analysis

P. Albuquerque, Baptista, A. J., Derengowsky, Lda Silveir, Procópio, L., Nicola, A. Moraes, Arraes, F. B. M., de Souza, D. Paulo, Kyaw, C. Maria, and Silva-Pereira, I., Paracoccidioides brasiliensis RNA biogenesis apparatus revealed by functional genome analysis, vol. 4, pp. 251-272, 2005.

The RNA biogenesis machinery of Paracoccidioides brasiliensis was assessed by comparative analyses of PbAESTs (P. brasiliensis assembled expressed sequence tags (ESTs)) with sequences from Saccharomyces cerevisiae MIPS database. PbAESTs related to almost all categories of S. cerevisiae RNA biogenesis were found. Two of the 12 S.

Virulence insights from the Paracoccidioides brasiliensis transcriptome

A. Henrique Tavares, Silva, S. Souza, Bernardes, V. Vilmar, Maranhão, A. Queiróz, Maranhão, A. Queiróz, Kyaw, C. Maria Kyaw, Poças-Fonseca, M., and Silva-Pereira, I., Virulence insights from the Paracoccidioides brasiliensis transcriptome, vol. 4. pp. 372-389, 2005.

Paracoccidioides brasiliensis, the etiologic agent of paracoccidioidomycosis, is a dimorphic fungus, which is found as mycelia at 22-26ºC and as yeasts at 37ºC. A remarkable feature common to several pathogenic fungi is their ability to differentiate from mycelium to yeast morphologies, or vice-versa. Although P. brasiliensis is a recognized pathogen for humans, little is known about its virulence genes. In this sense, we performed a search for putative virulence genes in the P. brasiliensis transcriptome.

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