Transcriptome

Transcriptome sequencing and analysis of the coconut leaf beetle, Brontispa longissima

W. Yan, Liu, L., Li, C. X., Huang, S. C., Ma, Z. L., Qin, W. Q., and Peng, Z. Q., Transcriptome sequencing and analysis of the coconut leaf beetle, Brontispa longissima, vol. 14, pp. 8359-8365, 2015.

The coconut leaf beetle, Brontispa longissima, is a de­structive pest of palm plants. Although its ecological and biological characteristics are well understood, its genetic information remains largely unknown. To advance our understanding of its molecular ba­sis of biology and ecology, we sequenced and analyzed its whole tran­scriptome by using high-throughput Illumina paired-end sequencing technology. Approximately 8.08 Gb of clean reads were generated in a single run, which were assembled by using Trinity into 41,652 unigenes with an average length of 932 bp.

Development and characterization of genic-SSR markers from different Asia lotus (Nelumbo nucifera) types by RNA-seq

X. F. Zheng, You, Y. N., Diao, Y., Zheng, X. W., Xie, K. Q., Zhou, M. Q., Hu, Z. L., and Wang, Y. W., Development and characterization of genic-SSR markers from different Asia lotus (Nelumbo nucifera) types by RNA-seq, vol. 14, pp. 11171-11184, 2015.

Nelumbo nucifera is an important economic vegetable and traditional medicine, but available genetic resources remain limited. Next generation sequencing has proven to be a rapid and effective means of identifying genic simple sequence repeat (genic-SSR) markers. This study developed genic-SSRs for N. nucifera using Illumina sequencing technology to assess diversity across cultivated and wild lotus. A total of 105,834 uni-contigs were produced with an average read length of 722 bp. Exactly 11,178 genic-SSR loci were identified in 9523 uni-contigs.

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