Detection of Toxoplasma gondii DNA in naturally infected sheep’s milk
“Detection of Toxoplasma gondii DNA in naturally infected sheep’s milk”, vol. 14, pp. 8658-8662, 2015.,
The objective of this study was to verify whether Toxoplasma gondii is excreted in the milk of naturally infected sheep. In order to accomplish this, 275 lactating ewes were used; these were bred extensively in 17 estates distributed across nine cities. Polymerase chain reaction amplification was used to detect T. gondii DNA in milk samples, and the indirect immunofluorescence test was employed for the detection of anti-T. gondii IgG antibodies in the sera, with a cut-off value of 1:64.