Total RNA extraction

Evaluation of the semen swim-up method for bovine sperm RNA extraction

C. M. Han, Chen, R., Li, T., Chen, X. L., Zheng, Y. F., Ma, M. T., Gao, Q. H., Han, C. M., Chen, R., Li, T., Chen, X. L., Zheng, Y. F., Ma, M. T., and Gao, Q. H., Evaluation of the semen swim-up method for bovine sperm RNA extraction, vol. 15, p. -, 2016.

Isolation of high-quality RNA is important for assessing sperm gene expression, and semen purification methods may affect the integrity of the isolated RNA. This study evaluated the effectiveness of the sperm swim-up method for seminal RNA isolation. Frozen semen samples in straws from three bulls of proven fertility were purified by the swim-up method. RNA extraction was carried out using the E.Z.N.A.TM Total RNA kit II, with non-swim-up sperm as a control.

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