Total RNA extraction

Efficient oil palm total RNA extraction with a total RNA extraction kit

S. H. Habib, Saud, H. M., and Kausar, H., Efficient oil palm total RNA extraction with a total RNA extraction kit, vol. 13, pp. 2359-2367, 2014.

Oil palm tissues are rich in polyphenols, polysaccharides and secondary metabolites; these can co-precipitate with RNA, causing problems for downstream applications. We compared two different methods (one conventional and a kit-based method - Easy-BlueTM Total RNA Extraction Kit) to isolate total RNA from leaves, roots and shoot apical meristems of tissue culture derived truncated leaf syndrome somaclonal oil palm seedlings. The quality and quantity of total RNA were compared through spectrophotometry and formaldehyde gel electrophoresis.

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