Tomato

Agrobacterium-mediated transformation of tomato with the ICE1 transcription factor gene

J. X. Juan, Yu, X. H., Jiang, X. M., Gao, Z., Zhang, Y., Li, W., Duan, Y. D., and Yang, G., “Agrobacterium-mediated transformation of tomato with the ICE1 transcription factor gene”, vol. 14, pp. 597-608, 2015.

ICE1 genes play a very important role in plants in cold conditions. To improve the cold resistance of tomato, the ICE1 gene of Arabidopsis thaliana was used to construct the plant expression vector p3301-ICE1, and was overexpressed in tomato through Agrobacterium-mediated transformation. Five strains of resistant plants were obtained. PCR and half-quantitative results showed that the ICE1 gene was transferred to tomato; three strains tested positive.

A novel random amplified polymorphic DNA-based strategy for genetic diversity analysis and identification of tomatoes

X. Cao, Wu, Z., Zhou, R., Jiang, F. L., and Yang, Z. E., “A novel random amplified polymorphic DNA-based strategy for genetic diversity analysis and identification of tomatoes”, vol. 14, pp. 1650-1661, 2015.

Cultivar identification diagrams (CIDs) provide a rapid and efficient approach for identifying cultivars based on random amplified polymorphic DNA (RAPD) markers. In this paper, 64 tomato cultivars were identified using a CID. Using RAPD profiles, clustering analysis was performed to analyze genetic diversity. The results showed that 8 RAPD primers could completely separate the 64 cultivars according to the obtained polymorphic bands; a CID of the 64 tomato cultivars was then constructed.

Effects of exogenous 5-aminolevulinic acid on photosynthesis, stomatal conductance, transpiration rate, and PIP gene expression of tomato seedlings subject to salinity stress

Y. Y. Zhao, Yan, F., Hu, L. P., Zhou, X. T., Zou, Z. R., and Cui, L. R., “Effects of exogenous 5-aminolevulinic acid on photosynthesis, stomatal conductance, transpiration rate, and PIP gene expression of tomato seedlings subject to salinity stress”, vol. 14, pp. 6401-6412, 2015.

The effects of exogenous 5-aminolevulinic acid (ALA) on photosynthesis, plant growth, and the expression of two aquaporin genes in tomato seedlings under control and salinity conditions were investigated. Exogenous ALA application significantly improved net photosynthetic rate (Pn), total chlorophyll content, and plant biomass accumulation of tomato seedlings under salinity stress.

Identification and expression analysis of YABBY family genes associated with fruit shape in tomato (Solanum lycopersicum L.)

H. Q. Han, Liu, Y., Jiang, M. M., Ge, H. Y., and Chen, H. Y., “Identification and expression analysis of YABBY family genes associated with fruit shape in tomato (Solanum lycopersicum L.)”, vol. 14, pp. 7079-7091, 2015.

YABBY family genes play important roles in the development of leaf, flower, and fruit. The purpose of this research was to integrate all the YABBY genes and analyze the correlation between gene expression and fruit shape in tomato. Scanning of 24 genomes of sequenced species demonstrated that YABBY genes were very normal and stable in flowering plants except the seedless plants. Nine YABBY genes in tomato were computationally and experimentally characterized.

Relationships among lipid peroxidation, SOD enzyme activity, and SOD gene expression profile in Lycopersicum esculentum L. exposed to cold stress

S. S. Aydin, Büyük, İ., and Aras, S., “Relationships among lipid peroxidation, SOD enzyme activity, and SOD gene expression profile in Lycopersicum esculentum L. exposed to cold stress”, vol. 12, pp. 3220-3229, 2013.

The current study was designed to evaluate lipid peroxidation (via malondialdehyde) levels, the superoxide dismutase (SOD) gene expression profile, and SOD enzyme activity in tomato plants (Lycopersicum esculentum L.) subjected to different time periods of cold stress (control, 2, 4, 6, 8, and 10 days). Results revealed that maximum lipid peroxidation occurred in plants exposed to cold stress for 10 days, and SOD enzyme activity gradually increased with increasing exposure to cold stress.

Gene expression patterns of invertase gene families and modulation of the inhibitor gene in tomato sucrose metabolism

Y. L. Zhang, Zhang, A. H., and Jiang, J., “Gene expression patterns of invertase gene families and modulation of the inhibitor gene in tomato sucrose metabolism”, vol. 12, pp. 3412-3420, 2013.

Patterns of gene expression in the different types of sucrose metabolism in the tomato are highly variable and heritable. This genetic variation causes considerable functional differences. We examined the patterns of expression of invertase (Inv) gene families and an invertase inhibitor (INH) gene involved in elongating roots, hypocotyls, and fruit of the tomato (Lycopersicon esculentum cv. Micro-Tom and L. chmielewskii) through a real-time quantitative PCR analysis.

Genetic analysis of NaCl tolerance in tomato

A. Saeed, Shahid, M. Q., Anjum, S. A., Khan, A. A., Shakeel, A., Saleem, M. F., and Saeed, N., “Genetic analysis of NaCl tolerance in tomato”, vol. 10, pp. 1754-1776, 2011.

We attempted to find the suitable parents for the development of tomato hybrids for high salt soils by exploiting combining ability, gene action and heterosis. Six salt-tolerant and three salt-intolerant genotypes, along with their 18 F1 crosses, were evaluated at seedling stage under 10 and 15 dS/m (NaCl) salinity stress, compared to the control level of salinity.

Diallel analysis of production traits among domestic, exotic and mutant germplasms of Lycopersicon

G. Pratta, Picardi, L. A., and Zorzoli, R., “Diallel analysis of production traits among domestic, exotic and mutant germplasms of Lycopersicon”, vol. 2, pp. 206-213, 2003.

The effects of wild germplasm on tomato fruit shelf life have not yet been completely evaluated. Three different genotypes of Lycopersicon esculentum (a cultivated variety, a homozygote for nor and a homozygote for rin), LA1385 of L. esculentum var. cerasiforme, LA722 of L. pimpinellifolium, and 10 diallel hybrids were assayed. Mean values of fruit shelf life, weight, shape, and mean number of flowers per cluster were analyzed after Griffing (1956, Aust. J. Biology 9: 463-493), method 2, model 1.

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