Temperature

Real-time PCR evaluation of cell-free DNA subjected to various storage and shipping conditions

Q. Wang, Cai, Y., Brady, P., and Vermeesch, J. R., Real-time PCR evaluation of cell-free DNA subjected to various storage and shipping conditions, vol. 14, pp. 12797-12804, 2015.

In this study, we attempted to explore the factors affecting the yield of cell-free fetal DNA (cffDNA) obtained from maternal blood samples, including the use of different types of collection tubes, the interval between sample processing, and sample shipping under extreme weather conditions. Blood samples were drawn into K3EDTA tubes and cell-stabilizing tubes (Streck blood collection tube, BCT) from women pregnant with male fetuses.

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