Targeting silence

Effect of specific silencing of EMMPRIN on the growth and cell cycle distribution of MCF-7 breast cancer cells

X. Q. Yang, Yang, J., Wang, R., Zhang, S., Tan, Q. W., Lv, Q., Meng, W. T., Mo, X. M., and Li, H. J., Effect of specific silencing of EMMPRIN on the growth and cell cycle distribution of MCF-7 breast cancer cells, vol. 14, pp. 15730-15738, 2015.

The extracellular matrix metalloproteinase inducer (EMMPRIN, CD147) is a member of the immunoglobulin family and shows increased expression in tumor cells. We examined the effect of RNAi-mediated EMMPRIN gene silencing induced by lentiviral on the growth and cycle distribution of MCF-7 breast cancer cells. Lentiviral expressing EMMPRIN-short hairpin RNA were packaged to infect MCF-7 cells. The inhibition efficiency of EMMPRIN was validated by real-time fluorescent quantitation polymerase chain reaction and western blotting.

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