Effect of specific silencing of EMMPRIN on the growth and cell cycle distribution of MCF-7 breast cancer cells
“Effect of specific silencing of EMMPRIN on the growth and cell cycle distribution of MCF-7 breast cancer cells”, vol. 14, pp. 15730-15738, 2015.
, The extracellular matrix metalloproteinase inducer (EMMPRIN, CD147) is a member of the immunoglobulin family and shows increased expression in tumor cells. We examined the effect of RNAi-mediated EMMPRIN gene silencing induced by lentiviral on the growth and cycle distribution of MCF-7 breast cancer cells. Lentiviral expressing EMMPRIN-short hairpin RNA were packaged to infect MCF-7 cells. The inhibition efficiency of EMMPRIN was validated by real-time fluorescent quantitation polymerase chain reaction and western blotting.