Swine

Comparative study of leptin and leptin receptor gene expression in different swine breeds

S. E. Georgescu, Manea, M. A., Dinescu, S., and Costache, M., “Comparative study of leptin and leptin receptor gene expression in different swine breeds”, vol. 13, pp. 7140-7148, 2014.

Leptin is an important regulator of appetite, energy metabolism, and reproduction and is mainly synthesized in the adipocytes and then secreted into the bloodstream. The leptin receptor was classified as type I cytokine receptor due to its structural homology with IL-6 receptors and the signaling pathways in which they are both involved.

Supplemental leucine and isoleucine affect expression of cationic amino acid transporters and myosin, serum concentration of amino acids, and growth performance of pigs

M. Cervantes-Ramírez, Mendez-Trujillo, V., Araiza-Piña, B. A., Barrera-Silva, M. A., González-Mendoza, D., and Morales-Trejo, A., “Supplemental leucine and isoleucine affect expression of cationic amino acid transporters and myosin, serum concentration of amino acids, and growth performance of pigs”, vol. 12, pp. 115-126, 2013.

Leucine (Leu) participates in the activity of cationic amino acid (aa) transporters. Also, branched-chain aa [Leu, isoleucine (Ile), and valine (Val)] share intestinal transporters for absorption. We conducted an experiment with 16 young pigs (body weight of about 16 kg) to determine whether Leu and Ile affect expression of aa transporters b0,+ and CAT-1 in the jejunum and expression of myosin in muscle, as well as serum concentration of essential aa, and growth performance in pigs.

Association of T1740C polymorphism of L-FABP with meat quality traits in Junmu No. 1 white swine

Y. H. Zhang, Dai, L. S., Ma, T. H., Wang, S. Z., Guo, J., Li, F. J., Zhang, S. M., Sun, B. X., Liu, D. F., Gao, Y., and Zhang, J. B., “Association of T1740C polymorphism of L-FABP with meat quality traits in Junmu No. 1 white swine”, vol. 12, pp. 235-241, 2013.

This study was designed to investigate a single nucleotide polymorphism in intron 1 of the liver fatty acid-binding protein (L-FABP) gene in 156 Junmu No. 1 white swine using PCR-single-strand conformational polymorphism. The association between the polymorphism and meat quality traits was also studied. The cloning and sequencing results indicated that the polymorphism in intron 1 was due to a T→C mutation at position 1740 of L-FABP, yielding three genotypes (TT, TC, and CC).

Construction of suppressor of cytokine signaling 2 (SOCS2) adenoviral overexpression vector and its impact on growth-hormone-induced lipolysis in swine primary adipocytes

H. L. Yang, Yan, J., Feng, M., Tan, X., Yan, G. Y., Gan, L., and Sun, C., “Construction of suppressor of cytokine signaling 2 (SOCS2) adenoviral overexpression vector and its impact on growth-hormone-induced lipolysis in swine primary adipocytes”, vol. 12, pp. 1283-1293, 2013.

We investigated the effect of overexpression suppressor of cytokine signaling 2 (SOCS2) on lipolysis in swine primary adipocytes (pAd) induced by growth hormone (GH). We constructed pAd-SOCS2 adenoviral overexpression vectors to infect HEK293 cells for virus packaging and propagation. Cultured swine primary adipocytes were infected with virus particles; after 48 h the infected adipocytes were treated with 500 ng GH/mL in the growth medium. Lipometabolism-related gene expressions were detected at 0, 0.25, 0.5, 1, 2, and 4 h, by measuring mRNA and protein levels.

Influence of the halothane gene (HAL) on pork quality in two commercial crossbreeds

A. C. P. Silveira, Freitas, P. F. A., César, A. S. M., César, A. S. M., Antunes, R. C., Guimarães, E. C., Batista, D. F. A., and Torido, L. C., “Influence of the halothane gene (HAL) on pork quality in two commercial crossbreeds”, vol. 10, pp. 1479-1489, 2011.

We evaluated the effect of the halothane (HAL) gene on the quality of pork in domestic pigs. Half-carcasses from two different commercial pig (Sus domestica) crossbreeds were analyzed, 46 of which were homozygous dominant (HALNN) and 69 of which were heterozygous (HALNn) for the halothane gene. The measures included backfat thickness, lean meat percentage, carcass weight, pH 24 h after slaughtering, color, and drip loss; DNA was extracted from the haunch muscle.

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