Survivin

Induction function of siRNA-mediated survivin gene silencing on nasopharyngeal carcinoma cell apoptosis

S. M. Fu, Tu, Z. H., Deng, L. Q., Cai, J. H., Liang, Z., Lin, Z. Q., and Wang, Y. T., “Induction function of siRNA-mediated survivin gene silencing on nasopharyngeal carcinoma cell apoptosis”, vol. 14, pp. 2537-2545, 2015.

We examined the function of survivin gene expression in patients with nasopharyngeal carcinoma (NPC), as well as small interfering RNA (siRNA) on controlling CNE-2 NPC proliferation and apoptosis. Immunohistological methods, in situ hybridization, and reverse transcription-polymerase chain reaction technique were used to detect survivin protein and mRNA expression. We designed an siRNA sequence to inhibit survivin gene expression. The MTT method was used to examine the function of siRNA on controlling cell growth and proliferation.

Effects of AFP gene silencing on Survivin mRNA expression inhibition in HepG2 cells

Z. L. Fang, Fang, N., Han, X. N., Huang, G., Fu, X. J., Xie, G. S., Wang, N. R., and Xiong, J. P., “Effects of AFP gene silencing on Survivin mRNA expression inhibition in HepG2 cells”, vol. 14, pp. 3184-3190, 2015.

We investigated the effects of alpha-fetoprotein (AFP) gene silencing on Survivin expression in HepG2 cells. Small interfering RNA technology was used to downregulate AFP expression in HepG2 cells. An enzyme-linked immunosorbent assay was used to measure AFP concentration in the supernatant before and after transfection. An MTT assay was used to detect cell proliferation activity before and after transfection.

Association of cyclin D1 and survivin expression with sensitivity to radiotherapy in patients with nasopharyngeal carcinoma

S. M. Fu, Xu, M. X., Lin, S. M., Liang, Z., and Cai, J. H., “Association of cyclin D1 and survivin expression with sensitivity to radiotherapy in patients with nasopharyngeal carcinoma”, vol. 13, pp. 3502-3509, 2014.

The association between cyclin D1 and survivin protein expressions with radiotherapy sensitivity in patients with nasopharyngeal carcinoma was investigated. Biopsy specimens of 72 patients with nasopharyngeal carcinoma were collected before the initiation of radiotherapy (49 cases were in the radiation-sensitive group and 23 cases were in the radiation-insensitive group). Conventional hematoxylin and eosin staining was used for tissue typing. The immunohistochemical SP method was used to detect cyclin D1 and survivin protein expression levels.

Inhibitory effect of survivin-targeting small interfering RNA on gastric cancer cells

Y. H. Li, Chen, M., Zhang, M., Zhang, X. Q., Zhang, S., Yu, C. G., Xu, Z. M., and Zou, X. P., “Inhibitory effect of survivin-targeting small interfering RNA on gastric cancer cells”, vol. 13, pp. 6786-6803, 2014.

A pair of inverted repeated sequences of the gene survivin was designed for stable double-stranded RNA establishment. After stable transfection, the biological behaviors of gastric cancer cells were observed. The interference rates of survivin-targeting siRNA (siRNA-survivin) in BGC823, MKN45, SGC7901, and cisplatin-resistant SGC7901 groups were 55.363 ± 3.974, 71.433 ± 3.774, 69.433 ± 7.336, and 76.767 ± 3.541%, respectively, compared with those in the control group.

Correlation of tumor relapse and elevated expression of survivin and vascular endothelial growth factor in superficial bladder transitional cell carcinoma

Y. W. Sun, Xuan, Q., Shu, Q. A., Wu, S. S., Chen, H., Xiao, J., Xiang, P., Zhu, Y. P., Wang, F. L., and Zhao, S. T., “Correlation of tumor relapse and elevated expression of survivin and vascular endothelial growth factor in superficial bladder transitional cell carcinoma”, vol. 12, pp. 1045-1053, 2013.

Survivin and vascular endothelial growth factor (VEGF) are newly discovered tumor markers closely correlated with bladder cancer. We analyzed the expression of survivin and VEGF in paraffin-embedded tumor tissues from 78 patients with bladder transitional cell carcinoma (BTCC) using an immunohistochemistry method. Normal bladder mucosae from 10 non-BTCC cases were also included as a control group. All patients were closely followed up for tumor recurrence after undergoing transurethral resection of bladder tumor procedures.

Association between survivin gene promoter -31G/C and -644C/T polymorphisms and non-small cell lung cancer

E. Aynaci, Coskunpinar, E., Eren, A., Kum, O., Oltulu, Y. M., Akkaya, N., Turna, A., Yaylim, I., and Yildiz, P., “Association between survivin gene promoter -31G/C and -644C/T polymorphisms and non-small cell lung cancer”, vol. 12, pp. 3975-3982, 2013.

Lung cancer is the most common cancer worldwide. Survivin is one of the first reported inhibitors of apoptosis proteins, which is an important family of proteins that regulate apoptosis. The survivin gene is located on human chromosome 17q25, which is composed of 142 amino acids. A common polymorphism of the survivin gene promoter -31G/C has been shown to influence cancer risk. This genetic variant has been associated with overexpression of survivin at both protein and mRNA levels in cancer cells.

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