Suppression subtractive hybridization

Identification of genes involved in spontaneous leaf color variation in Pseudosasa japonica

H. Y. Yang, Xia, X. W., Fang, W., Fu, Y., An, M. M., and Zhou, M. B., Identification of genes involved in spontaneous leaf color variation in Pseudosasa japonica, vol. 14, pp. 11827-11840, 2015.

Spontaneous leaf color variation in bamboo provides the opportunity to study the mechanisms of leaf color formation and the breeding of ornamental bamboos. Despite the fact that many genes are known to be involved in leaf color variation in model plants, molecular mechanisms governing natural leaf color variation in bamboo have remained obscure. This study aimed to identify the genes responsible for the occurrence of such phenomena in bamboo using the suppression subtractive hybridization (SSH) method between green and albino leaves in Pseudosasa japonica f.

Screening of genes related to ovarian development in the swimming crab, Portunus trituberculatus, by suppression subtractive hybridization

Z. B. Yu, Mu, C. K., Song, W. W., Li, R. H., Chen, Y. E., and Wang, C. L., Screening of genes related to ovarian development in the swimming crab, Portunus trituberculatus, by suppression subtractive hybridization, vol. 14, pp. 18675-18686, 2015.

The swimming crab, Portunus trituberculatus, is an important marine animal and is widely cultured in China. In the present study, suppression subtractive hybridization was applied to identify the differentially expressed genes in the ovaries of mature and immature P. trituberculatus. One hundred and seventy six expressed sequence tag (ESTs) were identified, of which 100 were down-regulated, and 76 up-regulated. BLAST analysis identified 51 unigenes, of which 27 were down-regulated, and 24 up-regulated.

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