Cloning of superoxide dismutase from post-harvest Hami melon and quantitative expression analysis before and after disease
“Cloning of superoxide dismutase from post-harvest Hami melon and quantitative expression analysis before and after disease”, vol. 14, pp. 18229-18240, 2015.
, Primers were designed according to the Cu/Zn-SOD gene sequences of cloned Cucurbits plants (cucumbers and watermelons) available in NCBI. Total RNA from Hami melon pulp was used as a template. Following RT-PCR amplification, a 403-bp fragment of the Hami melon Cu/Zn-SOD gene was obtained. According to alignment in BLAST and phylogenetic tree analysis, the cloned gene fragment was confirmed to be the Hami melon Cu/Zn-SOD gene sequence.