Sugarcane

Identification of smut-responsive genes in sugarcane using cDNA-SRAP

N. Huang, Zhang, Y. Y., Xiao, X. H., Huang, L., Wu, Q. B., Que, Y. X., and Xu, L. P., Identification of smut-responsive genes in sugarcane using cDNA-SRAP, vol. 14, pp. 6808-6818, 2015.

Sugarcane smut, caused by the fungus Sporisorium scitamineum, is one of the main diseases that affect sugarcane worldwide. In the present study, the cDNA-SRAP technique was used to identify genes that are likely to be involved in the response of sugarcane to S. scitamineum infection. In total, 21 bands with significant differential expression during cDNA-SRAP analysis were cloned and sequenced.

Genetic variability among the chloroplast genomes of sugarcane (Saccharum spp) and its wild progenitor species Saccharum spontaneum L.

J. - R. Zhu, Zhou, H., Pan, Y. - B., and Lu, X., Genetic variability among the chloroplast genomes of sugarcane (Saccharum spp) and its wild progenitor species Saccharum spontaneum L., vol. 13, pp. 3037-3047, 2014.

A striking characteristic of modern sugarcane is that all sugarcane cultivars (Saccharum spp) share a common cytoplasm from S. officinarum. To explore the potential value of S. spontaneum cytoplasm, new Saccharum hybrids with an S. spontaneum cytoplasm were developed at the United States Department of Agriculture-Agricultural Research Service, Sugarcane Research Laboratory, through a combination of conventional and molecular breeding approaches.

Genetic diversity among Puccinia melanocephala isolates from Brazil assessed using simple sequence repeat markers

R. F. Peixoto-Junior, Creste, S., Landell, M. G. A., Nunes, D. S., Sanguino, A., Campos, M. F., Vencovsky, R., Tambarussi, E. V., and Figueira, A., Genetic diversity among Puccinia melanocephala isolates from Brazil assessed using simple sequence repeat markers, vol. 13, pp. 7852-7863, 2014.

Brown rust (causal agent Puccinia melanocephala) is an important sugarcane disease that is responsible for large losses in yield worldwide. Despite its importance, little is known regarding the genetic diversity of this pathogen in the main Brazilian sugarcane cultivation areas. In this study, we characterized the genetic diversity of 34 P. melanocephala isolates from 4 Brazilian states using loci identified from an enriched simple sequence repeat (SSR) library.

Use of differential levels of mean observed heterozygosity in microsatellite loci of commercial varieties of sugarcane (Saccharum spp)

R. C. Maranho, Augusto, R., Mangolin, C. A., and Machado, M. F. P. S., Use of differential levels of mean observed heterozygosity in microsatellite loci of commercial varieties of sugarcane (Saccharum spp), vol. 13, pp. 10130-10141, 2014.

In this study, we measured the genetic diversity within and among a set of 9 commercial sugarcane varieties used for alcohol and sugar production using 17 microsatellite DNA markers. The UGSM148 and UGSM59 primers were monomorphic for all 74 sugarcane samples. The estimated proportion of simple sequence repeated (SSR) polymorphic loci was 88.23%; 17 alleles were detected. The mean gene diversity of all SSR loci was 0.7279.

Establishment of an in vitro regeneration system for genetic transformation of selected sugarcane genotypes

S. Ijaz, Rana, I. A., Khan, I. A., and Saleem, M., Establishment of an in vitro regeneration system for genetic transformation of selected sugarcane genotypes, vol. 11, pp. 512-530, 2012.

A good culture system provides considerable quantities of highly regenerable target tissues. Embryogenic callus cultures are ideal for micro-projectile-mediated transformation, because regenerable cells are not very stable. Effective exploitation of genetic transformation requires good regeneration systems. We selected three sugarcane genotypes for the establishment and optimization of good in vitro regeneration systems, viz., S-2003-us-359, S-2006-sp-30, and S-2003-us-165. Three callus induction media were investigated.

Use of simple sequence repeat markers for DNA fingerprinting and diversity analysis of sugarcane (Saccharum spp) cultivars resistant and susceptible to red rot

U. Hameed, Pan, Y. - B., Muhammad, K., Afghan, S., and Iqbal, J., Use of simple sequence repeat markers for DNA fingerprinting and diversity analysis of sugarcane (Saccharum spp) cultivars resistant and susceptible to red rot, vol. 11, pp. 1195-1204, 2012.

Red rod is an economically important disease of sugarcane caused by the fungus Colletotrichum falcatum. We used a simple sequence repeat (SSR)-based marker system to identify and analyze genetic relationships of red rot resistant and susceptible sugarcane cultivars grown in Pakistan. Twenty-one highly polymorphic SSR markers were used for DNA fingerprinting and genetic diversity analysis of 20 sugarcane cultivars. These SSR markers were found to be highly robust; we identified 144 alleles, with 3-11 alleles per marker and a mean of 6.8.

Molecular characterization of endophytes isolated from Saccharum spp based on esterase and ribosomal DNA (ITS1-5.8S-ITS2) analyses

A. C. Leme, Bevilaqua, M. R. R., Rhoden, S. A., Mangolin, C. A., Machado, M. F. P. S., and Pamphile, J. A., Molecular characterization of endophytes isolated from Saccharum spp based on esterase and ribosomal DNA (ITS1-5.8S-ITS2) analyses, vol. 12, pp. 4095-4105, 2013.

This study used esterases and ribosomal DNA (rDNA) markers to determine endophytic variability in order to better understand endophyte-host interactions. Polyacrylamide gel electrophoresis and esterase isoenzymes (EST; EC 3.1.1.3), with α-naphthyl acetate and β-naphthyl acetate as substrates, were used to assess relationships among endophytes. ITS1-5.8S-ITS2 sequencing data were used as rDNA markers. Thirty-two esterases were obtained from 37 isolates of Saccharum spp, which clustered into five endophyte groups.

Culture-independent analysis of endophytic bacterial communities associated with Brazilian sugarcane

G. S. Magnani, Cruz, L. M., Weber, H., Bespalhok, J. C., Daros, E., Baura, V., Yates, M. G., Monteiro, R. A., Faoro, H., Pedrosa, F. O., and Souza, E. M., Culture-independent analysis of endophytic bacterial communities associated with Brazilian sugarcane, vol. 12, pp. 4549-4558, 2013.

Sugarcane is an economically important culture in Brazil. Endophytic bacteria live inside plants, and can provide many benefits to the plant host. We analyzed the bacterial diversity of sugarcane cultivar RB-72454 by cultivation-independent techniques. Total DNA from sugarcane stems from a commercial plantation located in Paraná State was extracted. Partial 16S rRNA genes were amplified and sequenced for library construction. Of 152 sequences obtained, 52% were similar to 16S rRNA from Pseudomonas sp, and 35.5% to Enterobacter sp.

DNA profiling of sugarcane genotypes using randomly amplified polymorphic DNA

S. Tabasum, Khan, F. A., Nawaz, S., Iqbal, M. Z., and Saeed, A., DNA profiling of sugarcane genotypes using randomly amplified polymorphic DNA, vol. 9, pp. 471-483, 2010.

DNA profiles of 40 sugarcane genotypes were constructed with 30 RAPD markers. Sugarcane genotypes of both Saccharum officinarum and S. barberi were included in this study. Multiple alleles were detected from each RAPD; there was a high level of polymorphism. On average, 7.93 alleles were produced per primer, giving a total of 238 alleles. The genetic distances between these genotypes were assessed with the POPGENE DNA sequence analysis software. A dendrogram was constructed from these data; cultivated species of sugarcane formed clusters with S.

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