Subunit bacterin

Protective efficacy of a genetic subunit bacterin against edema disease of swine in mice

X. L. Yang, Liu, Y. J., Hu, L. Q., Guo, L. W., Liu, G. P., Wu, B., and Chen, H. C., Protective efficacy of a genetic subunit bacterin against edema disease of swine in mice, vol. 14, pp. 18026-18033, 2015.

The exotoxin SLT-IIeB from the Escherichia coli Ee strain was expressed in E. coli, and the recombinant protein was purified, mixed with the Ee strain, then emulsified with oil-emulsion adjuvants to obtain a mixed subunit bacterin. Groups of Kunming mice were immunized at weeks 0 and 2, and challenged intraperitoneally with the Ee strain at week 4. Antibodies were detected by ELISA and an agglutination test.

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