Protective efficacy of a genetic subunit bacterin against edema disease of swine in mice
“Protective efficacy of a genetic subunit bacterin against edema disease of swine in mice”, vol. 14, pp. 18026-18033, 2015.
, The exotoxin SLT-IIeB from the Escherichia coli Ee strain was expressed in E. coli, and the recombinant protein was purified, mixed with the Ee strain, then emulsified with oil-emulsion adjuvants to obtain a mixed subunit bacterin. Groups of Kunming mice were immunized at weeks 0 and 2, and challenged intraperitoneally with the Ee strain at week 4. Antibodies were detected by ELISA and an agglutination test.