Sos recruitment system for the analysis of the interaction between sulfatase-modifying factor 2 subtypes and interleukin-13
“Sos recruitment system for the analysis of the interaction between sulfatase-modifying factor 2 subtypes and interleukin-13”, vol. 12, pp. 5664-5672, 2013.
, Interleukin (IL)-13 is a central mediator in allergic asthma. Our previous results have indicated that sulfatase-modifying factor 2 (SUMF2) interacts with IL-13 and inhibits its secretion. In this study, we investigated the interactions between SUMF2 subtypes and 2 types of IL-13. Wild type IL-13 (wh-IL-13) and its mutated counterpart (mh-IL-13) were analyzed and cloned into pSos yeast expression vectors. Protein was expressed in host cdc25H yeast strains.