Subtractive hybridization

Construction and analysis of a subtractive cDNA library of early embryonic development in duck

Y. L. Liu, Zhong, L. X., Li, J. J., Shen, J. D., Wang, D. Q., Tao, Z. R., Shi, F. X., and Lu, L. Z., Construction and analysis of a subtractive cDNA library of early embryonic development in duck, vol. 12, pp. 2234-2247, 2013.

Several studies have documented the process of early embryonic development in poultry; however, the molecular mechanisms underlying its developmental regulation are poorly understood, particularly in ducks. In this study, we analyzed differential gene expression of embryos 6 and 25 h following oviposition to determine which genes regulate the early developmental stage in ducks. Among 216 randomly selected clones, 39 protein-encoding cDNAs that function in metabolism, transcription, transportation, proliferation/apoptosis, cell cycle, cell adhesion, and methylation were identified.

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