SSRs

Isolation and characterization of polymorphic microsatellite loci from aerial yam (Dioscorea bulbifera L.)

Q. Q. Yan, Li, Y., Sun, X. Q., Guo, J. L., Hang, Y. Y., and Li, M. M., Isolation and characterization of polymorphic microsatellite loci from aerial yam (Dioscorea bulbifera L.), vol. 13, pp. 1514-1517, 2014.

Dioscorea bulbifera L. is widely distributed in pantropical regions along the equator. The taxonomic treatment of this species is ambiguous due to its extreme polymorphic morphological characters. In order to provide tools to facilitate the study of genetic diversity, population structure, patterns of gene flow, and the mating system of this species, and to assess intraspecific variability and relationships in D. bulbifera, 14 novel polymorphic microsatellite loci were developed using the dual-suppression PCR technique.

Molecular characterization of the Andean blackberry, Rubus glaucus, using SSR markers

M. Marulanda, López, A. M., and Uribe, M., Molecular characterization of the Andean blackberry, Rubus glaucus, using SSR markers, vol. 11, pp. 322-331, 2012.

The species Rubus glaucus, also known as the Andean or “Castilla” blackberry, is one of nine edible species of this genus that grow naturally in Central and South America. In Colombia, this species is the most important of all Rubus species for agricultural and commercial purposes. We used 20 SSRs developed for other Rubus species to characterize 44 Colombian R. glaucus genotypes, collected from eight different departments, and to look for molecular differences between thornless and thorny cultivated blackberries.

Genetic diversity analysis of Bt cotton genotypes in Pakistan using simple sequence repeat markers

I. Ullah, Iram, A., Iqbal, M. Z., Nawaz, M., Hasni, S. M., and Jamil, S., Genetic diversity analysis of Bt cotton genotypes in Pakistan using simple sequence repeat markers, vol. 11, pp. 597-605, 2012.

The popularity of genetically modified insect resistant (Bt) cotton has promoted large scale monocultures, which is thought to worsen the problem of crop genetic homogeneity. Information on genetic diversity among Bt cotton varieties is lacking. We evaluated genetic divergence among 19 Bt cotton genotypes using simple sequence repeat (SSR) markers. Thirty-seven of 104 surveyed primers were found informative.

Microsatellite markers for genetic studies of the fall armyworm, Spodoptera frugiperda

V. A. C. Pavinato, Martinelli, S., de Lima, P. F., Zucchi, M. I., and Omoto, C., Microsatellite markers for genetic studies of the fall armyworm, Spodoptera frugiperda, vol. 12. pp. 370-380, 2013.

We developed six microsatellite markers for the fall armyworm Spodoptera frugiperda (Lepidoptera: Noctuidae). The SSR loci were isolated with enriched genomic library protocol by using native individuals as a genome source for markers. These loci were characterized in 48 individuals and they were tested for the ability to identify candidate migrants exchanged among the samples. The number of alleles per locus ranged from 5 to 18 (10.8 on average). The observed polymorphism information content ranged from 0.172 to 0.891.

Comparison of simple sequence repeats in 19 Archaea

S. Trivedi, Comparison of simple sequence repeats in 19 Archaea, vol. 5, pp. 741-772, 2006.

All organisms that have been studied until now have been found to have differential distribution of simple sequence repeats (SSRs), with more SSRs in intergenic than in coding sequences. SSR distribution was investigated in Archaea genomes where complete chromosome sequences of 19 Archaea were analyzed with the program SPUTNIK to find di- to penta-nucleotide repeats. The number of repeats was determined for the complete chromosome sequences and for the coding and non-coding sequences.

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