SSR

Assessment of genetic diversity of Tunisian orange, Citrus sinensis (L.) Osbeck using microsatellite (SSR) markers

A. Mahjbi, Oueslati, A., Baraket, G., Salhi-Hannachi, A., S. Azouzi, Z., Mahjbi, A., Oueslati, A., Baraket, G., Salhi-Hannachi, A., and S. Azouzi, Z., Assessment of genetic diversity of Tunisian orange, Citrus sinensis (L.) Osbeck using microsatellite (SSR) markers, vol. 15, p. -, 2016.

Citrus are one of the most cultivated crops in the world. Economically, they are very important fruit trees in Tunisia. Little is known about the genetic diversity of the Tunisian Citrus germplasm. Exploring this diversity is a prerequisite for the identification and characterization of the local germplasm to circumvent and controlling genetic erosion caused by biotic and abiotic stress to aid its conservation and use.

Development of microsatellite markers in Hagenia abyssinica (Bruce) J.F. Gmel, an endangered tropical tree of eastern Africa, using next-generation sequencing

A. W. Gichira, Long, Z. C., Hu, G. W., Gituru, R. W., Wang, Q. F., Chen, J. M., Gichira, A. W., Long, Z. C., Hu, G. W., Gituru, R. W., Wang, Q. F., and Chen, J. M., Development of microsatellite markers in Hagenia abyssinica (Bruce) J.F. Gmel, an endangered tropical tree of eastern Africa, using next-generation sequencing, vol. 15, p. -, 2016.

Hagenia abyssinica (Bruce) J.F. Gmel is an endangered tree species endemic to the high mountains of tropical Africa. We used Illumina paired-end technology to sequence its nuclear genome, aiming at creating the first genomic data library and developing the first set of genomic microsatellites. Seventeen microsatellite markers were validated in 24 individuals. The average number of alleles per locus was 7.6, while the observed and expected heterozygosities ranged from 0.000 to 0.958 and from 0.354 to 0.883, respectively.

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