SSR

Genetic relationships of the Japanese persimmon Diospyros kaki (Ebenaceae) and related species revealed by SSR analysis

D. L. Guo and Luo, Z. R., Genetic relationships of the Japanese persimmon Diospyros kaki (Ebenaceae) and related species revealed by SSR analysis, vol. 10, pp. 1060-1068, 2011.

Simple sequence repeat (SSR) molecular markers based on 18 primers were employed to study the genetic relationship of Japanese persimmon (Diospyros kaki) specimens. Two hundred and sixty-two bands were detected in 30 Japanese persimmon samples, including 14 Japanese and 10 Chinese genotypes of Japanese persimmon (Diospyros kaki) and six related species, D. lotus, D. glaucifolia, D. oleifera, D. rhombifolia, D. virginiana, and Jinzaoshi (unclassified - previously indicated to be D. kaki).

Heterologous amplification and characterization of microsatellite markers in the Neotropical fish Leporinus friderici

A. M. Olivatti, Boni, T. A., Silva-Júnior, N. J., Resende, L. V., Gouveia, F. O., and Telles, M. P. C., Heterologous amplification and characterization of microsatellite markers in the Neotropical fish Leporinus friderici, vol. 10. pp. 1403-1408, 2011.

Leporinus friderici, native to the Amazon Basin and popularly known as “piau-três-pintas”, has great ecological and economic importance; it is widely fished and consumed throughout much of tropical South America. Knowledge of the genetic diversity of this native species is important to support management and conservation programs. We evaluated microsatellite loci amplification, using heterologous primers, in 31 individuals of L. friderici.

Variability of red rot-resistant somaclones of sugarcane genotype S97US297 assessed by RAPD and SSR

M. T. H. Shahid, Khan, F. A., Saeed, A., and Fareed, I., Variability of red rot-resistant somaclones of sugarcane genotype S97US297 assessed by RAPD and SSR, vol. 10, pp. 1831-1849, 2011.

Sugarcane breeding under climatic conditions of Pakistan is very difficult due to unavailability of viable fuzz (seed). Somaclonal variation can provide an alternative for improvement of existing genotypes. Six hundred and twenty-seven somaclones were developed from sugarcane genotype S97US297, and protocols for callogenesis and organogenesis were developed using Murashige and Skoog medium. Two types of explants, leaf and pith, and two auxins, 2,4-dichlorophenoxy acetic acid and indole-3-acetic acid, were tested to optimize callogenesis for root establishment.

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