SSCP

Molecular identification of Amazonian stingless bees using polymerase chain reaction single-strand conformation polymorphism

M. T. Souza and Carvalho-Zilse, G. A., “Molecular identification of Amazonian stingless bees using polymerase chain reaction single-strand conformation polymorphism”, vol. 13, pp. 5507-5513, 2014.

In countries containing a mega diversity of wildlife, such as Brazil, identifying and characterizing biological diversity is a continuous process for the scientific community, even in face of technological and scientific advances. This activity demands initiatives for the taxonomic identification of highly diverse groups, such as stingless bees, including molecular analysis strategies. This type of bee is distributed in all of the Brazilian states, with the highest species diversity being found in the State of Amazônia.

Polymorphisms in the ovine myostatin gene are associated with birth weight but not with weight gain in Iranian Makoei sheep

M. Farhadian, Hashemi, A., Mardani, K., Darvishzadeh, R., and Jafari, S., “Polymorphisms in the ovine myostatin gene are associated with birth weight but not with weight gain in Iranian Makoei sheep”, vol. 11, pp. 3568-3575, 2012.

Myostatin, a transforming growth factor-beta superfamily member, has been well documented as a negative regulator of muscle growth and development. Myostatin, which has 376 amino acids, is synthesized as a precursor protein. Polymorphism of the myostatin gene in Makoei sheep was investigated by PCR and single-strand conformation polymorphism technique (SSCP). Genomic DNA of 92 sheep was isolated from whole blood. A 417-bp myostatin intron I segment was amplified by standard PCR, using locus-specific primers.

Genetic diversity of wild and domesticated stocks of Thai abalone, Haliotis asinina (Haliotidae), analyzed by single-strand conformational polymorphism of AFLP-derived markers

P. Praipue, Klinbunga, S., and Jarayabhand, P., “Genetic diversity of wild and domesticated stocks of Thai abalone, Haliotis asinina (Haliotidae), analyzed by single-strand conformational polymorphism of AFLP-derived markers”, vol. 9, pp. 1136-1152, 2010.

Amplified fragment length polymorphism (AFLP) analysis was carried out on representative individuals of wild Haliotis asinina using 64 primer combinations. Nine polymorphic AFLPs were cloned and sequenced. Sequence-specific primers were designed from six AFLP-derived fragments. Three sequence-characterized amplified region (SCAR) markers (HaSCAR320, HaSCAR295, HaSCAR327) were selected for genotyping of 8-month-old domesticated stocks of H.

Development of a species-diagnostic marker and its application for population genetics studies of the stingless bee Trigona collina in Thailand

M. Theeraapisakkun, Klinbunga, S., and Sittipraneed, S., “Development of a species-diagnostic marker and its application for population genetics studies of the stingless bee Trigona collina in Thailand”, vol. 9, pp. 919-930, 2010.

A molecular maker for authenticating species origin of the stingless bee (Trigona collina) was developed. Initially, amplified fragment length polymorphism analysis was made of 11 stingless bee species using 64 primer combinations. A 316-bp band found only in T. collina was cloned and sequenced. A primer pair (CUTc1-F/R) was designed and tested for species-specificity in 15 stingless bee species (239 nests). The expected 259-bp fragment was consistently amplified in all T. collina individuals (134/134 nests, 100%).

Analysis of polymorphism based on SSCP markers in gamma-irradiated (Co60) grape (Vitis vinifera) varieties

D. D. Karataş, Kunter, B., Coppola, G., and Velasco, R., “Analysis of polymorphism based on SSCP markers in gamma-irradiated (Co60) grape (Vitis vinifera) varieties”, vol. 9, pp. 2357-2363, 2010.

The effects of induced mutation produced by five different doses of gamma irradiation (20, 25, 30, 40, and 45 Gy) were determined using molecular approaches in Vitis vinifera cultivars, namely Thompson Seedless (Sultani Çekirdeksiz) (progenitor of seedless vinifera variety) and Kalecik Karası (one of the best quality wine grape variety of Turkey). Mutant candidates were selected through morphological observations of mutation-induced phenotypic changes during the first, second and third vegetation periods after radiation applications.

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