SRY gene

Isolation and analysis of cell-free fetal DNA from maternal peripheral blood in Chinese women

W. C. Yang, Zhu, L., Qiu, Y. M., Zhou, B. X., Cheng, J. L., Wei, C. L., Chen, H. C., Li, L. Y., Fu, X. D., and Fu, J. J., Isolation and analysis of cell-free fetal DNA from maternal peripheral blood in Chinese women, vol. 14, pp. 18078-18089, 2015.

Non-invasive prenatal diagnosis is used to detect the genetic material of the fetus by isolating the cell-free fetal DNA (cffDNA) from maternal peripheral blood. In order to establish an isolation method for cffDNA from maternal peripheral blood in Chinese women, the cffDNA was acquired with a two-step centrifugation using a QlAamp DNA Blood mini kit. The SRY gene of plasma DNA was amplified by polymerase chain reaction (PCR). Real-time quantitative PCR was used to measure the concentration of cffDNA in maternal peripheral blood in different pregnant women.

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