DNA barcoding, which was developed about a decade ago, relies on short, standardized regions of the genome to identify plant and animal species. This method can be used to not only identify known species but also to discover novel ones. Numerous sequences are stored in online databases worldwide. One of the ways to save cost and time (by omitting the sequencing step) in species identification is to use available barcode data to design optimized primers for further analysis, such as high-resolution melting analysis (HRM).
“Identification of Uvaria sp by barcoding coupled with high-resolution melting analysis (Bar-HRM)”, vol. 15, p. -, 2016.,
“Development of primer pairs from diverse chloroplast genomes for use in plant phylogenetic research”, vol. 14, pp. 14857-14870, 2015.,
Variation in the chloroplast DNA sequence is useful for plant phylogenetic studies. However, the number of variable sequences provided by chloroplast DNA for suggested genes or genomic regions in plant phylogenetic analyses is often inadequate.