Soybean regeneration

Construction and analysis of a suppression subtractive hybridization library of regeneration-related genes in soybean

J. Sun, Li, J., Liu, M., Zhang, B. B., Li, D. M., Wang, M., Zhang, C., Li, W. B., Su, A. Y., and Wu, X. X., Construction and analysis of a suppression subtractive hybridization library of regeneration-related genes in soybean, vol. 14, pp. 763-773, 2015.

The development of a genetic transformation system is needed to address the problem of the low efficiency associated with soybean regeneration. To contribute to the enhancement of the soybean regenerative capacity, we explored the developmental mechanisms of soybean regeneration at the molecular level using a suppression subtractive hybridization cDNA library constructed from cotyledonary nodes of soybean cultivar DN50. A total of 918 positive clones were identified and screened, with most inserted fragments ranging from 100 to 750 bp.

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