Soybean

Construction and analysis of a suppression subtractive hybridization library of regeneration-related genes in soybean

J. Sun, Li, J., Liu, M., Zhang, B. B., Li, D. M., Wang, M., Zhang, C., Li, W. B., Su, A. Y., and Wu, X. X., Construction and analysis of a suppression subtractive hybridization library of regeneration-related genes in soybean, vol. 14, pp. 763-773, 2015.

The development of a genetic transformation system is needed to address the problem of the low efficiency associated with soybean regeneration. To contribute to the enhancement of the soybean regenerative capacity, we explored the developmental mechanisms of soybean regeneration at the molecular level using a suppression subtractive hybridization cDNA library constructed from cotyledonary nodes of soybean cultivar DN50. A total of 918 positive clones were identified and screened, with most inserted fragments ranging from 100 to 750 bp.

Genome-wide identification and expression analysis of the CPP-like gene family in soybean

L. Zhang, Zhao, H. K., Wang, Y. M., Yuan, C. P., Zhang, Y. Y., Li, H. Y., Yan, X. F., Li, Q. Y., and Dong, Y. S., Genome-wide identification and expression analysis of the CPP-like gene family in soybean, vol. 14, pp. 1260-1268, 2015.

Cysteine-rich polycomb-like protein (CPP-like) genes are a group of transcription factors with highly conserved cysteine-rich domains and are widely distributed in animals and plants, but do not present in yeast. Previous studies have shown that members of this family play important roles in the development of reproductive tissue and in the control of cell division in plants. In this study, whole genome identification of soybean CPP transcription factors was performed using bioinformatic methods.

Positive selection sites in tertiary structure of Leguminosae Chalcone isomerase 1

R. K. Wang, Zhan, S. F., Zhao, T. J., Zhou, X. L., and Wang, C. E., Positive selection sites in tertiary structure of Leguminosae Chalcone isomerase 1, vol. 14, pp. 1957-1967, 2015.

Isoflavonoids and the related synthesis enzyme, chalcone isomerase 1 (CHI1), are unique in the Leguminosae, with diverse biological functions. Among the Leguminosae, the soybean is an important oil, protein crop, and model plant. In this study, we aimed to detect the generation pattern of Leguminosae CHI1. Genome-wide sequence analysis of CHI in 3 Leguminosae and 3 other closely related model plants was performed; the expression levels of soybean chalcone isomerases were also analyzed.

Cloning and expression analysis of a stress-induced GmIMT1 gene in soybean (Glycine max)

H. T. Wang, Guo, N., Zhao, J. M., Karthikeyan, A., Xue, D., Xue, C. C., Xu, J. Y., Xu, Z. H., Gai, J. Y., and Xing, H., Cloning and expression analysis of a stress-induced GmIMT1 gene in soybean (Glycine max), vol. 13, pp. 806-818, 2014.

Here, we aimed to clone and identify the GmIMT1 gene related to the salt stress response in soybean. The full-length cDNA sequence of the GmIMT1 gene was amplified in soybean using degenerate primers of Mesembrythmum crystallium. To understand the stress response, the GmIMT1 gene was cloned and sequenced. Then, the expression vectors of the gene were constructed, and introduced into the model plant Arabidopsis thaliana through Agrobacterium mediated transformation, and the salt tolerance was analyzed in the transgenic plants.

Detection of genetically modified maize and soybean in feed samples

S. Meriç, Çakır, Ö., Turgut-Kara, N., and Arı, Ş., Detection of genetically modified maize and soybean in feed samples, vol. 13, pp. 1160-1168, 2014.

Despite the controversy about genetically modified (GM) plants, they are still incrementally cultivated. In recent years, many food and feed products produced by genetic engineering technology have appeared on store shelves. Controlling the production and legal presentation of GM crops are very important for the environment and human health, especially in terms of long-term consumption. In this study, 11 kinds of feed obtained from different regions of Turkey were used for genetic analysis based on foreign gene determination.

Quantative trait loci of seed traits for soybean in multiple environments

J. Y. Che, Ding, J. J., Liu, C. Y., Xin, D. W., Jiang, H. W., Hu, G. H., and Chen, Q. S., Quantative trait loci of seed traits for soybean in multiple environments, vol. 13, pp. 4000-4012, 2014.

Seed length and seed width are an important factor to the soybean yield. So the quantitative trait loci (QTL) location for seed length and seed width could assistant the breeding of soybean. In this study, the QTL underlying seed length and seed width were studied. A recombinant inbred line population of soybeans derived from a cross between the American semi-draft cultivars Charleston and Dongnong 594 were used in 7 environments. The quantitative trait loci underlying seed length, seed width, and seed length/seed width were analyzed by the method of composite interval mapping.

Genetic analysis of the major gene plus polygene model in soybean resistance to Leguminivora glycinivorella

J. Zhang, Yao, D., Ma, J., Fu, Y. - P., Qu, J., and Wang, P. - W., Genetic analysis of the major gene plus polygene model in soybean resistance to Leguminivora glycinivorella, vol. 13, pp. 4983-4989, 2014.

In order to investigate the genetic characteristics of soybean Leguminivora glycinivorella resistance and to improve soybean resistance insectivorous breeding efficiency by applying the multi-generation joint analysis method of the major gene plus polygene model, 5 pedigrees and generations (P1, F1, P2, F2, and F2:3) were used as the materials to perform the soybean L. glycinivorella resistance multi-generation joint analysis. The results showed that soybean resistance to L.

Cloning and functional prediction of differentially expressed genes in the leaves of Glycine max parents and hybrids at the seedling stage

J. Zhang, Yao, D., Wang, P., Guan, S. Y., Ma, J., and Fu, Y. P., Cloning and functional prediction of differentially expressed genes in the leaves of Glycine max parents and hybrids at the seedling stage, vol. 13, pp. 5474-5483, 2014.

Here, we compare the molecular mechanism of soybean heterosis through the differential expression of basic cloning. Specifically, we cloned 22 differentially expressed cDNA fragments from hybrid combinations of Jilin 38 x EXP (which had obvious yield advantages) and their parents. In addition, we compared the homology of these fragments and predicted their functions.

Overexpression of the activated form of the AtAREB1 gene (AtAREB1ΔQT) improves soybean responses to water deficit

J. P. Leite, Barbosa, E. G. G., Marin, S. R. R., Marinho, J. P., Carvalho, J. F. C., Pagliarini, R. F., Cruz, A. S., Oliveira, M. C. N., Farias, J. R. B., Neumaier, N., Guimarães, F. C. M., Yoshida, T., Kanamori, N., Fujita, Y., Nakashima, K., Shinozaki, K. Y., Desidério, J. A., and Nepomuceno, A. L., Overexpression of the activated form of the AtAREB1 gene (AtAREB1ΔQT) improves soybean responses to water deficit, vol. 13, pp. 6272-6286, 2014.

Abscisic acid-responsive element binding protein (AREB1) is a basic domain/leucine zipper transcription factor that binds to the abscisic acid (ABA)-responsive element motif in the promoter region of ABA-inducible genes. Because AREB1 is not sufficient to direct the expression of downstream genes under non-stress conditions, an activated form of AREB1 (AREB1ΔQT) was created. Several reports claim that plants overexpressing AREB1 or AREB1ΔQT show improved drought tolerance.

Mapping an aphid resistance gene in soybean [Glycine max (L.) Merr.] P746

L. Xiao, Zhong, Y. P., Wang, B., and Wu, T. L., Mapping an aphid resistance gene in soybean [Glycine max (L.) Merr.] P746, vol. 13, pp. 9152-9160, 2014.

Soybean aphid (SA: Aphis glycines Matsumura) is one of the most serious pests of soybean [Glycine max (L.) Merr.] worldwide. A single dominant gene was found to control SA resistance in soybean line P746, which exhibits antibiosis resistance. This study aimed to define the location of the SA resistance gene in P746. A F2:3 mapping population, including 312 individuals, was created based on the cross of P746 and ‘Dongnong 47’.

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