Agrobacterium-mediated transformation of the β-subunit gene in 7S globulin protein in soybean using RNAi technology
“Agrobacterium-mediated transformation of the β-subunit gene in 7S globulin protein in soybean using RNAi technology”, vol. 15, p. -, 2016.,
The objective of this study was to use RNA interference (RNAi) to improve protein quality and decrease anti-nutritional effects in soybean. Agrobacterium tumefaciens-mediated transformation was conducted using RNAi and an expression vector containing the 7S globulin β-subunit gene. The BAR gene was used as the selective marker and cotyledonary nodes of soybean genotype Jinong 27 were chosen as explant material. Regenerated plants were detected by molecular biology techniques.