Solexa sequencing

Identification and abundance of miRNA in chicken hypothalamus tissue determined by Solexa sequencing

G. R. Sun, Li, M., Li, G. X., Tian, Y. D., Han, R. L., and Kang, X. T., Identification and abundance of miRNA in chicken hypothalamus tissue determined by Solexa sequencing, vol. 11, pp. 4682-4694, 2012.

We used Solexa sequencing technology to identify and determine the abundance of miRNAs and compared the characteristics and expression patterns of miRNA of 1-day-old and 36-week-old chicken hypothalamuses. We obtained 17,825,753 and 10,928,745 high-quality reads from 36-week-old and 1-day-old chickens, respectively. Three hundred and seventy-one conserved miRNAs were expressed in both libraries. Among the conserved miRNAs, 22 miRNAs were up-regulated and 157 miRNAs were down-regulated in the 36-week-old chicken hypothalamus tissues.

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