Intraspecific variability of popcorn S lines for phosphorus efficiency in the soil.
, “Intraspecific variability of popcorn S lines for phosphorus efficiency in the soil.”, Genet Mol Res, vol. 16, no. 3, 2017.
Soil
Construction and validation of metagenomic DNA libraries from landfarm soil microorganisms
, “Construction and validation of metagenomic DNA libraries from landfarm soil microorganisms”, vol. 12, pp. 2148-2155, 2013.
Landfarming biodegradation is a strategy used by the petrochemical industry to reduce pollutants in petroleum-contaminated soil. We constructed 2 metagenomic libraries from landfarming soil in order to determine the pathway used for mineralization of benzene and to examine protein expression of the bacteria in these soils. The DNA of landfarm soil, collected from Ilhéus, BA, Brazil, was extracted and a metagenomic library was constructed with the Copy ControlTM Fosmid Library Production Kit, which clones 25-45-kb DNA fragments.
An improved extraction protocol for metagenomic DNA from a soil of the Brazilian Atlantic Rainforest
, “An improved extraction protocol for metagenomic DNA from a soil of the Brazilian Atlantic Rainforest”, vol. 7, pp. 1226-1232, 2008.
The need for the prospecting for and identification of new biomolecules is a reality. Molecular techniques allow access to the metabolic potential of microorganisms via the isolation of DNA from environmental samples, i.e., without the application of microbial culture techniques. With its great biological diversity, the Atlantic Rainforest biome has a soil rich in organic matter, some components of which interfere negatively in the reactions necessary for the exploitation of its biotechnological potential.
